This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cullins (Cul) are components of SCF-type ubiquitin ligases implicated in signal transduction, cell cycle control, and differentiation. These diverse functions are thought to be mediated by ubiquitin-dependent destruction of critical regulatory proteins. While there is a plethora of information on Cul-1, very little is known about its family members. The primary focus of this project involves identifying interacting proteins of Cul-3 and Cul-4. It is also interesting to point out that these proteins have been implicated in disease and are required for murine viability. Because these proteins are conserved in yeast (pcu3 and pcu4), we have chosen it as a platform in order to carry out the purification of the endogenous complex. We have employed the tandem affinity purification method for both pcu3 and pcu4 and have been recently successful in obtaining unique bands for both proteins.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR011823-11
Application #
7420659
Study Section
Special Emphasis Panel (ZRG1-CB-H (40))
Project Start
2006-09-20
Project End
2007-08-31
Budget Start
2006-09-20
Budget End
2007-08-31
Support Year
11
Fiscal Year
2006
Total Cost
$2,859
Indirect Cost
Name
University of Washington
Department
Biochemistry
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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