This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In an effort to cure diabetes, one promising development is the implantation of gel encapsulated cells that can produce insulin. These encapsulated cells can then be implanted in patients. We are developing a variety of encapsulation methods and are using MR both on encapsulated cell samples and in vivo in animal models in order to evaluate different systems and methods. In particular, we are presently evaluating a variety of alginate combinations to generate alginate beads with different properties and characteristics and evaluating their ability to support different cell types. A publication describing this work on beads of differing alginate compositions has now been published. Additionally we continue to examine the MR characteristics of beads implanted with pancreatic islets. Most recently we obtained the first MR images of human islets. For these studies we have integrated the microcoils with a perfusion system to keep the tissues viable in the instrument. The images show a clear heterogeneity within the beads. We are in the process of reproducing these results and determining if they provide information relevant with respect to tissue viability. These data were used in the submission of an NIH grant on microimaging studies of the beads (PI = Dr Constantinidis).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR016105-05S1
Application #
7369578
Study Section
Special Emphasis Panel (ZRG1)
Project Start
2005-05-01
Project End
2007-04-30
Budget Start
2005-05-01
Budget End
2007-04-30
Support Year
5
Fiscal Year
2006
Total Cost
$5,792
Indirect Cost
Name
University of Florida
Department
Neurosciences
Type
Schools of Medicine
DUNS #
969663814
City
Gainesville
State
FL
Country
United States
Zip Code
32611
Flint, Jeremy J; Hansen, Brian; Blackband, Stephen J (2016) Diffusion tensor microscopy data (15.6 ?m in-plane) of white matter tracts in the human, pig, and rat spinal cord with corresponding tissue histology. Data Brief 9:271-4
Flint, Jeremy J; Hansen, Brian; Fey, Michael et al. (2010) Cellular-level diffusion tensor microscopy and fiber tracking in mammalian nervous tissue with direct histological correlation. Neuroimage 52:556-61
Tarver, Matthew R; Schmelz, Eric A; Rocca, James R et al. (2009) Effects of soldier-derived terpenes on soldier caste differentiation in the termite Reticulitermes flavipes. J Chem Ecol 35:256-64
Shepherd, Timothy M; Thelwall, Peter E; Stanisz, Greg J et al. (2009) Aldehyde fixative solutions alter the water relaxation and diffusion properties of nervous tissue. Magn Reson Med 62:26-34
Barmpoutis, Angelos; Jian, Bing; Vemuri, Baba C (2009) Adaptive kernels for multi-fiber reconstruction. Inf Process Med Imaging 21:338-49
Flint, Jeremy J; Lee, Choong H; Hansen, Brian et al. (2009) Magnetic resonance microscopy of mammalian neurons. Neuroimage 46:1037-40
Flint, Jeremy; Hansen, Brian; Vestergaard-Poulsen, Peter et al. (2009) Diffusion weighted magnetic resonance imaging of neuronal activity in the hippocampal slice model. Neuroimage 46:411-8
Shepherd, Timothy M; Flint, Jeremy J; Thelwall, Peter E et al. (2009) Postmortem interval alters the water relaxation and diffusion properties of rat nervous tissue--implications for MRI studies of human autopsy samples. Neuroimage 44:820-6
Constantinidis, Ioannis; Grant, Samuel C; Simpson, Nicholas E et al. (2009) Use of magnetic nanoparticles to monitor alginate-encapsulated betaTC-tet cells. Magn Reson Med 61:282-90
Collins, Christopher M (2009) Numerical field calculations considering the human subject for engineering and safety assurance in MRI. NMR Biomed 22:919-26

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