One goal of our Center is to determine the role of inhibitory neuronal systems and receptors in genetic differences in alcohol sensitivity. This research direction has arisen from our collective work showing differences between LS and SS mice in GABAa receptors. In this project, we propose to link and extend ongoing studies in the ARC by asking if any of five major QTLs for ethanol sensitivity determine functional differences in GABAa receptors. This approach will utilize congenic mice from component 3 for biochemical studies of GABAa receptor function. In addition, we will focus on the roles of the two variants of the gamma subunit of the GABAa receptor in alcohol sensitivity in vivo and in vitro. These studies will be based on transgenic mice that overexpress either the gamma2S or gamma2L subunit. Effects of these transgenes will be evaluated on several different genetic backgrounds including 129SvevTac, C57BL/6J, ILS, and ISS. These mice will also be provided to other projects in the ARC.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Specialized Center (P50)
Project #
5P50AA003527-23
Application #
6299165
Study Section
Project Start
1999-12-01
Project End
2000-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
23
Fiscal Year
2000
Total Cost
$150,034
Indirect Cost
Name
University of Colorado Denver
Department
Type
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
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