Core - Morphology

Kahn, Arnold

Abstract

This core will be responsible for preparing, sectioning and staining samples for routine histology, immuno- and enzyme histochemistry, and in situ hybridization. The core will also provide training to Center investigators and their associates in the use of microtomes, microscopes and in photomicrography. The core will consist of 60% support for a highly experienced SRA III under the supervision of Dr. Kahn. All projects will utilize this core extensively. It will be particularly essential for Project 5 in which tissue, rather than cell cultures, is used exclusively. This must be embedded and section in order to obtain any morphological information. Since Project 3 and 4 propose extensive use of the model for some of their proposed experiments, and since Projects 1 and 2 utilize tissue as well as cell cultures, this core is essential for all investigators.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Specialized Center (P50)
• Project #: 5P50DE010306-03
• Application #: 3732625
• Support Year: 3
• Fiscal Year: 1995

Institution

• Name: University of California San Francisco
• DUNS #: 073133571
• City: San Francisco
• State: CA
• Country: United States
• Zip Code: 94143

Publications

Kamiya, Nobuhiro; Jikko, Akitoshi; Kimata, Koji et al. (2002) Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. J Bone Miner Res 17:1832-42

Choy, L; Skillington, J; Derynck, R (2000) Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. J Cell Biol 149:667-82

Suen, P W; Ilic, D; Caveggion, E et al. (1999) Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages. J Cell Sci 112 ( Pt 22):4067-78

Johnson, P W; Lancero, H (1999) Function of gingival fibroblasts and periodontal ligament cells in the presence of methyl mercaptan. Quintessence Int 30:343-9

Gerber, H P; Vu, T H; Ryan, A M et al. (1999) VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nat Med 5:623-8

Bullard, K M; Lund, L; Mudgett, J S et al. (1999) Impaired wound contraction in stromelysin-1-deficient mice. Ann Surg 230:260-5

Filvaroff, E; Erlebacher, A; Ye, J et al. (1999) Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development 126:4267-79

Miettinen, P J; Chin, J R; Shum, L et al. (1999) Epidermal growth factor receptor function is necessary for normal craniofacial development and palate closure. Nat Genet 22:69-73

Jikko, A; Harris, S E; Chen, D et al. (1999) Collagen integrin receptors regulate early osteoblast differentiation induced by BMP-2. J Bone Miner Res 14:1075-83

Iwasaki, M; Jikko, A; Le, A X (1999) Age-dependent effects of hedgehog protein on chondrocytes. J Bone Joint Surg Br 81:1076-82