Core - Tissue Procurement

El-Naggar, Adel K.

Abstract

The tissue core facility will function as a repository for all patients specimens including peripheral blood lymphocytes that are required for the different research projects of the oral cancer research center. The facility will be responsible for acquisition, storage and distribution of histologically characterized samples from different lesions comprising the histopathological spectrum of oral squamous neoplasia. Detailed mapping of each specimen followed by a careful sampling and verification process to determine the nature and the extent of each lesion will be conducted on each sample. Standardized coding, processing, preservation and inventory of the specimens will be carried out to ensure uniform and standardized handling, to secure patient confidentiality and avert analytical bias. The core will also be responsible for centralized processing and extraction of protein, RNA, and DNA to maximize tissue utilization from small and limited specimens. Flow cytometric analysis of varying parameters including proliferation, Bcl-2 and apoptosis, as well as immunohistochemical staining for oncogenes, tumor suppressor genes, and cellular markers will also be performed as requested by specific projects.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Specialized Center (P50)
• Project #: 1P50DE011906-01
• Application #: 5210281
• Support Year: 1
• Fiscal Year: 1996

Publications

Wang, Heng; Hicks, John; Khanbolooki, Parham et al. (2003) Transgenic mice demonstrate novel promoter regions for tissue-specific expression of the urokinase receptor gene. Am J Pathol 163:453-64

Gonzalez, Hernan E; Gujrati, Manu; Frederick, Mitchell et al. (2003) Identification of 9 genes differentially expressed in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 129:754-9

Jayakumar, Arumugam; Kang, Ya'an; Frederick, Mitchell J et al. (2003) Inhibition of the cysteine proteinases cathepsins K and L by the serpin headpin (SERPINB13): a kinetic analysis. Arch Biochem Biophys 409:367-74

Chun, Kyung-Hee; Benbrook, Doris M; Berlin, K Darrell et al. (2003) The synthetic heteroarotinoid SHetA2 induces apoptosis in squamous carcinoma cells through a receptor-independent and mitochondria-dependent pathway. Cancer Res 63:3826-32

Higuchi, Eisaku; Chandraratna, Roshantha A S; Hong, Waun K et al. (2003) Induction of TIG3, a putative class II tumor suppressor gene, by retinoic acid in head and neck and lung carcinoma cells and its association with suppression of the transformed phenotype. Oncogene 22:4627-35

Liu, Yanna; Li, Jun Z; Yuan, Xiao H et al. (2002) An AP-1 binding site mutation in HPV-16 LCR enhances E6/E7 promoter activity in human oral epithelial cells. Virus Genes 24:29-37

Yan, Chunhong; Wang, Heng; Boyd, Douglas D (2002) ATF3 represses 72-kDa type IV collagenase (MMP-2) expression by antagonizing p53-dependent trans-activation of the collagenase promoter. J Biol Chem 277:10804-12

El-Naggar, Adel K; Kim, Hyung W; Clayman, Gary L et al. (2002) Differential expression profiling of head and neck squamous carcinoma: significance in their phenotypic and biological classification. Oncogene 21:8206-19

Shin, M; Yan, C; Boyd, D (2002) An inhibitor of c-jun aminoterminal kinase (SP600125) represses c-Jun activation, DNA-binding and PMA-inducible 92-kDa type IV collagenase expression. Biochim Biophys Acta 1589:311-6

Hayashi, K; Yokozaki, H; Naka, K et al. (2001) Overexpression of retinoic acid receptor beta induces growth arrest and apoptosis in oral cancer cell lines. Jpn J Cancer Res 92:42-50