The 'Mass Spectrometry and Imaging Technologies in Geroscience1 P30 Core will consist of an integratedsuite of mass spectrometry and microscopic imaging technologies coupled to specific protein and smallmolecule drug and metabolite analysis assays and bioenergetic analysis.
The aims of this P30 Core are tointegrate the unique instrumentation facilities at the Buck Institute in imaging and mass spectrometry totarget the needs of the 10 components of the U54 in structural and functional proteomics, and for theanalysis of drugs and targeted metabolites that constitute this Geroscience initiative. Specifically, theimaging and mass spectrometry technologies will encompass; (i) functional and fixed-cell imaging usingprotein-specific fluorophores and antibodies, (ii) functional analysis of intact neurons and other cells includingbioenergetic and redox measurements, (iii) development and implementation of protein chemistries, (iv)mass spectrometry-based analysis of proteins, including their identification, quantitation, interactions andposttranslational modifications, (v) mass spectrometry imaging and profiling studies, and (vi) drug andmetabolite analyses. These studies will be carried out on a range of aging and aging-related disease modelsthat constitute the components of this U54. The P30 core will consist of two main but integrated parts:'Functional and Structural Imaging Technologies' directed by Dr. Nicholls and 'Mass SpectrometryTechnologies' directed by the principal investigator, Dr. Bradford Gibson. We propose to apply and integratethese instrument-based technologies at the very earliest stages of experimental design and work closely withthe other investigators to develop, optimize, and implement these mass spectrometry and imagingtechnologies.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Linked Center Core Grant (PL1)
Project #
1PL1AG032118-01
Application #
7466745
Study Section
Special Emphasis Panel (ZRR1-SRC (99))
Program Officer
Sierra, Felipe
Project Start
2007-09-30
Project End
2012-06-30
Budget Start
2007-09-30
Budget End
2008-06-30
Support Year
1
Fiscal Year
2007
Total Cost
$834,828
Indirect Cost
Name
Buck Institute for Age Research
Department
Type
DUNS #
786502351
City
Novato
State
CA
Country
United States
Zip Code
94945
Debattisti, Valentina; Gerencser, Akos A; Saotome, Masao et al. (2017) ROS Control Mitochondrial Motility through p38 and the Motor Adaptor Miro/Trak. Cell Rep 21:1667-1680
Mark, Karla A; Dumas, Kathleen J; Bhaumik, Dipa et al. (2016) Vitamin D Promotes Protein Homeostasis and Longevity via the Stress Response Pathway Genes skn-1, ire-1, and xbp-1. Cell Rep 17:1227-1237
Nishida, Yuya; Rardin, Matthew J; Carrico, Chris et al. (2015) SIRT5 Regulates both Cytosolic and Mitochondrial Protein Malonylation with Glycolysis as a Major Target. Mol Cell 59:321-32
Rardin, Matthew J; Schilling, Birgit; Cheng, Lin-Yang et al. (2015) MS1 Peptide Ion Intensity Chromatograms in MS2 (SWATH) Data Independent Acquisitions. Improving Post Acquisition Analysis of Proteomic Experiments. Mol Cell Proteomics 14:2405-19
Quinlan, Casey L; Perevoschikova, Irina V; Goncalves, Renata L S et al. (2013) The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production. Methods Enzymol 526:189-217
Rardin, Matthew J; He, Wenjuan; Nishida, Yuya et al. (2013) SIRT5 regulates the mitochondrial lysine succinylome and metabolic networks. Cell Metab 18:920-33
Rardin, Matthew J; Newman, John C; Held, Jason M et al. (2013) Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways. Proc Natl Acad Sci U S A 110:6601-6
Quinlan, Casey L; Perevoshchikova, Irina V; Hey-Mogensen, Martin et al. (2013) Sites of reactive oxygen species generation by mitochondria oxidizing different substrates. Redox Biol 1:304-12
Brand, M D; Orr, A L; Perevoshchikova, I V et al. (2013) The role of mitochondrial function and cellular bioenergetics in ageing and disease. Br J Dermatol 169 Suppl 2:1-8
Choi, Sung W; Gerencser, Akos A; Ng, Ryan et al. (2012) No consistent bioenergetic defects in presynaptic nerve terminals isolated from mouse models of Alzheimer's disease. J Neurosci 32:16775-84

Showing the most recent 10 out of 55 publications