Mentored section: As part of the mentored project, the candidate would extend his published results of high-throughput multiantigen fluorescence microfluidic immunoassays (BioTechniques 40: 85-90) to more complex samples, such as human blood serum and plasma, thereby paving the way to portable microfluidic blood test systems. Relevance: Portable microfluidic blood tests would produce major savings in cost and time in modern biomedical diagnostics with concomitant savings in healthcare in general. Such systems would also use finger-prick amounts of sample, eliminating the need for phlebotomy and the danger of hematomas, as well as making blood tests more accessible to pediatric patients, particularly infants. Low costs and small amounts of sample would also mean more frequent and ubiquitous testing, resulting in earlier diagnosis and thus saving lives. Independent research section: The applicant has a detailed plan outlined further below and including microfluidic immunoassays for viral detection, nanodetector and microfluidics integration for portable diagnostic devices, devices for large-scale cellular screening, on-chip PCR machine for on-the-spot forensic analysis, nested bioarrays for nanomedicine chips, DNA intercalator-separator of unknown samples, and a microfluidic cellular transfector.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Transition Award (R00)
Project #
5R00EB007151-05
Application #
7896440
Study Section
Special Emphasis Panel (NSS)
Program Officer
Korte, Brenda
Project Start
2008-09-01
Project End
2012-08-31
Budget Start
2010-09-01
Budget End
2012-08-31
Support Year
5
Fiscal Year
2010
Total Cost
$244,996
Indirect Cost
Name
University of Southern California
Department
Pathology
Type
Schools of Medicine
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Chang, Hyun-Joo; Ye, Wubing; Kartalov, Emil P (2012) Quantitative modeling of the behaviour of microfluidic autoregulatory devices. Lab Chip 12:1890-6
Maltezos, George; Lee, John; Rajagopal, Aditya et al. (2011) Microfluidic blood filtration device. Biomed Microdevices 13:143-6
Lin, David H; Taylor, Clive R; French Anderson, W et al. (2010) Internally calibrated quantification of VEGF in human plasma by fluorescence immunoassays in disposable elastomeric microfluidic devices. J Chromatogr B Analyt Technol Biomed Life Sci 878:258-63
Chang, Hyun-Joo; Kim, Se-Heon; Lee, Yong-Hee et al. (2010) A photonic-crystal optical antenna for extremely large local-field enhancement. Opt Express 18:24163-77
Liu, John; Chen, Yan; Taylor, Clive R et al. (2009) Elastomeric microfluidic diode and rectifier work with Newtonian fluids. J Appl Phys 106:114311
Kartalov, Emil P; Lin, David H; Lee, David T et al. (2008) Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices. Electrophoresis 29:5010-6