The long-term goal of this project is to identify thalamocortical network mechanisms involved in consolidating experience-dependent plasticity in the visual system. Sleep has beneficial effects for processes dependent upon synaptic plasticity, such as memory consolidation. Recent studies have shown that cortical areas engaged by waking sensory experience are """"""""reactivated"""""""" during subsequent slow wave sleep (SWS), with local changes in electroencephalogram (EEG) oscillatory activity. Because these EEG oscillations are generated by rhythmic, synchronous firing of thalamic and cortical neurons, one untested hypothesis is that SWS thalamocortical activity leads to potentiation or depression of synaptic targets. Orientation-specific response potentiation (OSRP) in the mouse visual system involves potentiation of neuronal responses to visual stimuli of a specific orientation. OSRP is initiated by brief exposure to an oriented grating stimulus, and is consolidated """"""""offline"""""""" in the hours immediately following visual experience. My preliminary data suggest that thalamocortical spindle (7-14 Hz) activity during SWS may play a critical role in OSRP consolidation. In the mentored phase of the proposed award (Aim 1), I will: (a) test whether SWS and SWS spindle oscillations are required for OSRP, and (b) assess whether during consolidation, SWS spindles 1) activate thalamocortical connections in a non-specific manner, or 2) mediate """"""""reactivation"""""""" of thalamocortical connections in a manner consistent with prior visual experience. I will do this by recording ongoing activity and visual response properties in populations of neurons in the visual cortex and lateral geniculate nucleus of freely-behaving mice during baseline, waking visual experience, and a subsequent consolidation period of either: ad lib sleep, total sleep deprivation, rapid eye movement sleep (REM) deprivation, or selective interruption of SWS spindles. These studies will build upon the my prior research experience with multielectrode recording and data analysis, under the co-mentorship of Drs. Marcos Frank (my current postdoctoral advisor and an expert in the areas of sleep and visual cortex plasticity) and Diego Contreras (an expert in the areas of state-dependent thalamocortical network properties and network mechanisms involved in vision). During the mentored phase of the award, I will also develop expertise in using optogenetic techniques in combination with multielectrode recording in freely-behaving mice, in preparation for experiments outlined in Aim 2. In the independent phase of the award (Aim 2), I will use this combination of state of the art techniques to silence defined populations of thalamocortical, reticular thalamic, or corticothalamic neurons during particular states (wake, REM, or SWS), to test the necessity of thalamocortical activity within each state for OSRP consolidation. I hypothesize that generation and coordination of spindles by these neuronal populations during SWS is critical for this process. Together, these studies will reveal state-dependent network mechanisms necessary for consolidating plasticity following visual experience.

Public Health Relevance

The proposed studies will provide new insights into how sleep and wake states uniquely contribute to synaptic plasticity in the visual system. Because cognitive processes such as memory formation rely on similar plasticity mechanisms, findings from these experiments may ultimately lead to novel treatments for disorders where both cognition and sleep patterns are adversely affected - such as Alzheimer's disease, schizophrenia, and autism.

National Institute of Health (NIH)
National Eye Institute (NEI)
Research Transition Award (R00)
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Special Emphasis Panel (NSS)
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Steinmetz, Michael A
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University of Michigan Ann Arbor
Schools of Arts and Sciences
Ann Arbor
United States
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Clawson, Brittany C; Durkin, Jaclyn; Suresh, Aneesha K et al. (2018) Sleep Promotes, and Sleep Loss Inhibits, Selective Changes in Firing Rate, Response Properties and Functional Connectivity of Primary Visual Cortex Neurons. Front Syst Neurosci 12:40
Puentes-Mestril, Carlos; Aton, Sara J (2017) Linking Network Activity to Synaptic Plasticity during Sleep: Hypotheses and Recent Data. Front Neural Circuits 11:61
Durkin, Jaclyn; Aton, Sara J (2016) Sleep-Dependent Potentiation in the Visual System Is at Odds with the Synaptic Homeostasis Hypothesis. Sleep 39:155-9
Clawson, Brittany C; Durkin, Jaclyn; Aton, Sara J (2016) Form and Function of Sleep Spindles across the Lifespan. Neural Plast 2016:6936381
Dumoulin, Michelle C; Aton, Sara J; Watson, Adam J et al. (2015) Extracellular signal-regulated kinase (ERK) activity during sleep consolidates cortical plasticity in vivo. Cereb Cortex 25:507-15
Dumoulin Bridi, Michelle C; Aton, Sara J; Seibt, Julie et al. (2015) Rapid eye movement sleep promotes cortical plasticity in the developing brain. Sci Adv 1:e1500105
Gerstner, Jason R; Aton, Sara J; Heller, H Craig (2015) Waking up to the alarm: sleep, clocks, and making memory (s)tick. Front Syst Neurosci 9:65
Aton, Sara J; Suresh, Aneesha; Broussard, Christopher et al. (2014) Sleep promotes cortical response potentiation following visual experience. Sleep 37:1163-70
Aton, Sara J (2013) Set and setting: how behavioral state regulates sensory function and plasticity. Neurobiol Learn Mem 106:1-10
Aton, Sara J; Broussard, Christopher; Dumoulin, Michelle et al. (2013) Visual experience and subsequent sleep induce sequential plastic changes in putative inhibitory and excitatory cortical neurons. Proc Natl Acad Sci U S A 110:3101-6

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