Major advancesin human health will come from understanding the functions of the genome. Unfortunately, many phenotypes are too variable for genetic analyses. A phenotype isthe product of both genetic and environmentalfactors, and although the genetic ones can be controlled with great precision, the environmental ones cannot. This isbecause the pertinent environmentalvariables influencing many phenotypes have not been identified. This project isfocusedon two phenotypesthat are highly variable even in inbred mice;taste preferences and diet-induced obesity. Thegoal isto assess the contribution of some early life laboratory variables to these adult phenotypes. The early variables manipulated will be (1) litter size and composition. Thehypothesis underlying this aim is that one or more factor related to the mouse's interaction with itslittermates (both in and ex utero) produces life-long effects on itsphenotype. The approach will involve comparing the adult phenotypes of mice originating from litters of different sizes and sexratios. (2) Neonatal and weaning diet. The hypothesis underlying this aim isthat uncontrolled aspects of the diet mice ingest before and during weaning influences adult phenotype variability. The approach will be to compare the phenotypes of adult mice fed various diets whenyoung. (3) Factors acting during shipping from breeder to test institution. Thehypothesisunderlying this aim isthat factors related to the transportationof mice from breeder to test site are responsible for inducing variability in adult phenotype. The approach will be (a) monitor environmental parametersduring shipping, and (b) systematically evaluate the contribution of each environmental parameter (e.g., food deprivation, water deprivation, temperature, motion)on adult phenotype. Theproposed studies will characterizesome environmental factors that may exert uncontrolled effects on adult phenotypes. The results will lead to better environmental controls in future work and, asa direct consequence, easierand more rapid progress with genetic analyses.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Research Project (R01)
Project #
5R01AA012715-07
Application #
7534537
Study Section
Biobehavioral Regulation, Learning and Ethology Study Section (BRLE)
Program Officer
Noronha, Antonio
Project Start
2000-04-01
Project End
2009-11-30
Budget Start
2008-12-01
Budget End
2009-11-30
Support Year
7
Fiscal Year
2009
Total Cost
$199,465
Indirect Cost
Name
Monell Chemical Senses Center
Department
Type
DUNS #
088812565
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Antonarakis, Emmanuel S; Drake, Charles G (2012) Combining immunological and androgen-directed approaches: an emerging concept in prostate cancer immunotherapy. Curr Opin Oncol 24:258-65
Drake, Charles G; Antonarakis, Emmanuel S (2012) Current status of immunological approaches for the treatment of prostate cancer. Curr Opin Urol 22:197-202
Antonarakis, Emmanuel S; Drake, Charles G (2010) Current status of immunological therapies for prostate cancer. Curr Opin Urol 20:241-6
Tordoff, Michael G (2008) Gene discovery and the genetic basis of calcium consumption. Physiol Behav 94:649-59
Reed, Danielle R; Lawler, Maureen P; Tordoff, Michael G (2008) Reduced body weight is a common effect of gene knockout in mice. BMC Genet 9:4
Tordoff, Michael G (2007) Taste solution preferences of C57BL/6J and 129X1/SvJ mice: influence of age, sex, and diet. Chem Senses 32:655-71
McDaniel, Amanda H; Li, Xia; Tordoff, Michael G et al. (2006) A locus on mouse Chromosome 9 (Adip5) affects the relative weight of the gonadal but not retroperitoneal adipose depot. Mamm Genome 17:1078-92
Reed, Danielle R; McDaniel, Amanda H; Li, Xia et al. (2006) Quantitative trait loci for individual adipose depot weights in C57BL/6ByJ x 129P3/J F2 mice. Mamm Genome 17:1065-77
Tordoff, Michael G; Alarcon, Laura K; Byerly, Erica A et al. (2005) Mice acquire flavor preferences during shipping. Physiol Behav 86:480-6
Tordoff, Michael G; Bachmanov, Alexander A (2003) Mouse taste preference tests: why only two bottles? Chem Senses 28:315-24

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