Abstract

The ultimate goal of this project is to determine whether cultured lymphocytes represent a superior model for studying in vitro senescence. Since the aging immune system has been postulated as one of the causes of biological aging, lymphocytes are an eminently suitable, yet heretofore unexplored, model for studying senescence in vitro. T cell cultures and clones will be established from normal newborn and adult lymphocytes, and will be analyzed for growth, functional and cell surface characteristics. Any clones which show unlimited growth, with no signs of senescence, will be examined for signs of transformation. Thus, the biological question of whether normal lymphocytes conform to the Hayflick limit will be addressed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG005309-02
Application #
3115875
Study Section
Immunobiology Study Section (IMB)
Project Start
1985-09-30
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Effros, R B; Zhu, X; Walford, R L (1994) Stress response of senescent T lymphocytes: reduced hsp70 is independent of the proliferative block. J Gerontol 49:B65-70
Effros, R B; Boucher, N; Porter, V et al. (1994) Decline in CD28+ T cells in centenarians and in long-term T cell cultures: a possible cause for both in vivo and in vitro immunosenescence. Exp Gerontol 29:601-9
Perillo, N L; Naeim, F; Walford, R L et al. (1993) In vitro cellular aging in T-lymphocyte cultures: analysis of DNA content and cell size. Exp Cell Res 207:131-5
Vaziri, H; Schachter, F; Uchida, I et al. (1993) Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. Am J Hum Genet 52:661-7
Perillo, N L; Naeim, F; Walford, R L et al. (1993) The in vitro senescence of human T lymphocytes: failure to divide is not associated with a loss of cytolytic activity or memory T cell phenotype. Mech Ageing Dev 67:173-85
Perillo, N L; Walford, R L; Newman, M A et al. (1989) Human T lymphocytes possess a limited in vitro life span. Exp Gerontol 24:177-87
Hulette, C M; Effros, R B; Walford, R L (1987) Immunization of normal human splenocytes in vitro to produce human monoclonal antibodies to cellular antigens. Tissue Antigens 30:25-33
Effros, R B; Walford, R L (1987) Neonatal T cells as a model system to study the possible in vitro senescence of lymphocytes. Exp Gerontol 22:307-16
Effros, R B; Hulette, C M; Ettenger, R et al. (1986) A human-human hybridoma secreting anti-HLA class II antibody. J Immunol 137:1599-603