The overall objective is to obtain a better understanding of the regulation of the cholinergic innervation of the cerebral cortex. We propose experiments which are expected to provide answers to questions concerning the functional and anatomical organization of the cholinergic neurons in the Nucleus Basalis of Meynert and their relationships with the ventral strio-pallidal system. The planned studies will use the technique of intracellular recording IN VIVO to study the physiological properties of Nucleus Basalis neurons. The nature of afferent inputs will be studied by stimulation of respective areas. Moreover, we will utilize the technique of intracellular horseradish peroxidase labeling to examine the morphology and the synaptic relationships of the physiologically identified Nucleus Basalis neurons. We will also perform experiments involving histochemistry and/or immunocytochemistry on tissue containing intracellularly labeled neurons in order to determine the neurochemical properties of the labeled neurons and their synaptic partners. The multidisciplinary studies proposed in this project are unique to the ventral forebrain. The data will enhance our understanding of both the intrinsic functional organization of neurons in the Nucleus Basalis, and the nature of afferent regulations of cholinergic neurons which innervate the cortex. This information will be useful in the formulation of both preventive and therapeutic treatments of Alzheimer's Disease.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG005944-03
Application #
3116678
Study Section
Neurology A Study Section (NEUA)
Project Start
1986-09-01
Project End
1990-03-31
Budget Start
1988-09-01
Budget End
1990-03-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Tennessee Health Science Center
Department
Type
Schools of Medicine
DUNS #
941884009
City
Memphis
State
TN
Country
United States
Zip Code
38163
Herron, P; Baskerville, K A; Chang, H T et al. (1997) Distribution of neurons immunoreactive for parvalbumin and calbindin in the somatosensory thalamus of the raccoon. J Comp Neurol 388:120-9
Chang, H T; Tian, Q; Herron, P (1995) GABAergic axons in the ventral forebrain of the rat: an electron microscopic study. Neuroscience 68:207-20
Chang, H T (1993) Immunoperoxidase labeling of the anterograde tracer fluoro-ruby (tetramethylrhodamine-dextran amine conjugate). Brain Res Bull 30:115-8
Baskerville, K A; Chang, H T; Herron, P (1993) Topography of cholinergic afferents from the nucleus basalis of Meynert to representational areas of sensorimotor cortices in the rat. J Comp Neurol 335:552-62
Chang, H T; Kita, H (1992) Interneurons in the rat striatum: relationships between parvalbumin neurons and cholinergic neurons. Brain Res 574:307-11
Kuo, H; Chang, H T (1992) Ventral pallido-striatal pathway in the rat brain: a light and electron microscopic study. J Comp Neurol 321:626-36
Chang, H T (1991) Anterograde transport of lucifer yellow-dextran conjugate. Brain Res Bull 26:813-6
Chang, H T; Kuo, H (1991) Calcium-binding protein (calbindin D-28k) immunoreactive neurons in the basal forebrain of the monkey and the rat: relationship with the cholinergic neurons. Adv Exp Med Biol 295:119-26
Chang, H T; Kuo, H (1991) Relationship of calbindin D-28k and cholinergic neurons in the nucleus basalis of Meynert of the monkey and the rat. Brain Res 549:141-5
Chang, H T; Tian, Q (1991) Vasoactive intestinal polypeptide (VIP) immunoreactive elements in the caudal ventral striatum of the rat: a light and electron microscopic study. Brain Res Bull 26:947-56

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