During the early part of this century it was established that sunlight exposure was essential for the prevention and cure of rickets. Intensive investigations led to the concept that when skin is exposed to the UV-B portion of sunlight the 7-dehydrocholesterol stores partly convert to vitamin D3. However, very little is known regarding the sequential events that occur when cutaneous 7-dehydrocholesterol converts to vitamin D3. Our initial investigations into the photobiology of vitamin D3 have suggested that the skin is a unique organ responsible for the controlled synthesis and release of this important calcium hormone. We have established that during exposure to sunlight, epidermal 7-dehydrocholesterol is converted principally to previtamin D3 and not directly to vitamin D3. Once formed in the skin, this thermally labile previtamin slowly converts to vitamin D3 over a period of days. The vitamin D binding protein which has preferential affinity for vitamin D3 transports vitamin D3 through the circulation leaving the previtamin behind to continue its conversion to vitamin D3. During the past three years we have developed the necessary methodology including, (a) synthesis of radiolabeled 7-dehydrocholesterol, (b) non-cytotoxic skin separation techniques, and (c) a powerful high resolution high pressure liquid chromatographic system that directly analyzes the photoconversion of 7-dehydrocholesterol to previtamin D3 in human skin. Using these techniques we plan to investigate what influence age has on the ability of the skin to photosynthesize adequate quantities of vitamin D3. This will be accomplished by critically evaluating the effect age has upon, (a) the skin stores of 7-dehydrocholesterol, (b) the cutaneous photosynthesis of vitamin D3, (c) serum levels of vitamin D3 and its metabolites and (d) in vitro synthesis and metabolism of 7-dehydrocholesterol in human skin fibroblast and keratinocyte cultures.
| Webb, A R; Pilbeam, C; Hanafin, N et al. (1990) An evaluation of the relative contributions of exposure to sunlight and of diet to the circulating concentrations of 25-hydroxyvitamin D in an elderly nursing home population in Boston. Am J Clin Nutr 51:1075-81 |
| Holick, M F (1987) Photosynthesis of vitamin D in the skin: effect of environmental and life-style variables. Fed Proc 46:1876-82 |
| Holick, M F (1987) Vitamin D and the kidney. Kidney Int 32:912-29 |
