The cellular events leading to the pathogenesis of neurofibrillary pathology (NFP) in Alzheimer's disease (AD) are poorly understood. This proposal focuses on the pathogenic mechanisms at the cellular and subcellular level and focuses on immunoelectron microscopy of the lesions in well fixed brain tissue, obtained from either biopsy or rapid autopsy. We propose three studies which will provide significant new insights into the pathogenesis of NFP. First, the morphogenesis of abnormal filaments in the dystrophic neurites characteristic of AD will be studied by the accurate localization of cytoskeletal proteins in the different types of abnormal and normal filaments. In conjunction, studies will establish whether there is structural continuity between the abnormal filaments and the cytoskeleton. Further morphometric analysis will be used to determined the organization of the normal and abnormal filaments and whether neuropil threads originate from neurofibrillary tangle bearing neurons. Second, we will determine the chemical association of tubulin and the neurofilament heavy subunit with paired helical filaments (PHF). Identification of these proteins in enriched PHF fractions as well as quantification will be used to establish whether both are chemically integral PHF components. In a series of reconstitution experiments using neurofilament and microtubule proteins we will attempt to understand the factors responsible for abnormal filament formation in AD based on protein interaction. Finally, we will study the aged or alcohol treated rat as a model of NFP. Immunocytochemistry at the light and electron microscopic levels will be used to characterize the changes seen in rats and compared to NFP in AD. These results will serve as the prelude to protein chemical characterization of NFP in the rat model. These experiments are expected to provide significant information on a) the structural role the cytoskeleton plays in NFP formation as well as the interaction of normal and abnormal structures; b) identification of NFH and tubulin as components of NFP as well as their importance in abnormal filament formation; and c) development of a model to study NFP.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG009287-03
Application #
3121108
Study Section
Pathology A Study Section (PTHA)
Project Start
1990-09-01
Project End
1995-08-31
Budget Start
1992-09-01
Budget End
1993-08-31
Support Year
3
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
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Cuajungco, M P; Goldstein, L E; Nunomura, A et al. (2000) Evidence that the beta-amyloid plaques of Alzheimer's disease represent the redox-silencing and entombment of abeta by zinc. J Biol Chem 275:19439-42
Castellani, R J; Harris, P L; Lecroisey, A et al. (2000) Evidence for a novel heme-binding protein, HasAh, in Alzheimer disease. Antioxid Redox Signal 2:137-42
Nunomura, A; Perry, G; Pappolla, M A et al. (2000) Neuronal oxidative stress precedes amyloid-beta deposition in Down syndrome. J Neuropathol Exp Neurol 59:1011-7

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