Carbohydrate and Fsh Specificity Determination

Bousfield, George R.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute on Aging (NIA)
• Type: Research Project (R01)
• Project #: 5R01AG015428-06
• Application #: 6043105
• Study Section: Biochemical Endocrinology Study Section (BCE)
• Program Officer: Bellino, Francis
• Project Start: 1997-09-15
• Project End: 2001-07-31
• Budget Start: 1999-08-01
• Budget End: 2001-07-31
• Support Year: 6
• Fiscal Year: 1999

Institution

• Name: Wichita State University
• Department: Biology
• Type: Schools of Arts and Sciences
• City: Wichita
• State: KS
• Country: United States
• Zip Code: 67260

Publications

Bousfield, George R; Butnev, Vladimir Y; Butnev, Viktor Y et al. (2004) Differential effects of alpha subunit Asparagine56 oligosaccharide structure on equine lutropin and follitropin hybrid conformation and receptor-binding activity. Biochemistry 43:10817-33

Nguyen, Van T; Singh, Vinod; Butnev, Vladimir Y et al. (2003) Inositol phosphate stimulation by LH requires the entire alpha Asn56 oligosaccharide. Mol Cell Endocrinol 199:73-86

Butnev, V Y; Singh, V; Nguyen, V T et al. (2002) Truncated equine LH beta and asparagine(56)-deglycosylated equine LH alpha combine to produce a potent FSH antagonist. J Endocrinol 172:545-55

Walton, W J; Nguyen, V T; Butnev, V Y et al. (2001) Characterization of human FSH isoforms reveals a nonglycosylated beta-subunit in addition to the conventional glycosylated beta-subunit. J Clin Endocrinol Metab 86:3675-85

Bousfield, G R; Butnev, V Y; Butnev, V Y (2001) Identification of twelve O-glycosylation sites in equine chorionic gonadotropin beta and equine luteinizing hormone ss by solid-phase Edman degradation. Biol Reprod 64:136-47