The major goal of this research proposal is to examine the influence of age and gender on single muscle fiber contractile properties before and after 12-wks of progressive resistance training (PRT). Our work during the past three years (NIA AG15486) has provided insight to the cellular adaptations in 70-yr. old men and women before and after resistance training. However, data describing the contractile properties of single muscle fibers in men and women over 80 yr. is unknown. We have preliminary data showing that adults over 80 yr. have a continued reduction in contractile speed and that the fast-twitch (type II) fibers are selectively altered. This is an important finding that may help explain the accelerated muscle strength losses (approximately 30 percent per decade) observed after the age of 70 yr. In addition, single fiber contractile properties before and after PRT have not been characterized in young adults. Therefore, the intent of this research will be to extend our single muscle fiber studies to include young (20-30 yr) and very old (>80 yr) men and women. Before and after a 12-wk PRT program, type I (slow) and II (fast) muscle fibers will be analyzed for size (microm), peak force (Po), shortening velocity (Vo), force-velocity relationship, and power. We will test the hypotheses that: 1) There will be a progressive decline by age (young to very old) in the individual slow and fast-twitch single muscle fiber contractile function before and after PRT. This progressive decline in contractile function will be more pronounced in the fast-twitch muscle fibers (hypotheses 1-3), and 2) Gender differences in single fiber contractile function will be present in the older men and women, but not the younger men and women (hypothesis #4). Upon completion of this proposed research, along with our previous work on moderately old adults, we will have single muscle cell data before and after PRT in young (<30 yr), moderately old (approximately 70 yr), and very old (>80 yr) men and women. Together, these studies will provide a detailed understanding of sarcopenia at the cell level (i.e. fiber specific - slow vs. fast fibers) which are not detectable using whole muscle measurements. With this information we will be able to determine, for the first time, the interaction of aging, resistance training, and gender on single muscle fiber contractile function.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG018409-02
Application #
6536039
Study Section
Geriatrics and Rehabilitation Medicine (GRM)
Program Officer
Dutta, Chhanda
Project Start
2001-09-30
Project End
2006-08-31
Budget Start
2002-09-01
Budget End
2003-08-31
Support Year
2
Fiscal Year
2002
Total Cost
$286,000
Indirect Cost
Name
Ball State University
Department
Other Basic Sciences
Type
Schools of Arts and Sciences
DUNS #
065540726
City
Muncie
State
IN
Country
United States
Zip Code
47306
Raue, Ulrika; Trappe, Todd A; Estrem, Shawn T et al. (2012) Transcriptome signature of resistance exercise adaptations: mixed muscle and fiber type specific profiles in young and old adults. J Appl Physiol 112:1625-36
Williamson, David L; Raue, Ulrika; Slivka, Dustin R et al. (2010) Resistance exercise, skeletal muscle FOXO3A, and 85-year-old women. J Gerontol A Biol Sci Med Sci 65:335-43
Raue, Ulrika; Slivka, Dustin; Minchev, Kiril et al. (2009) Improvements in whole muscle and myocellular function are limited with high-intensity resistance training in octogenarian women. J Appl Physiol (1985) 106:1611-7
Slivka, Dustin; Raue, Ulrika; Hollon, Chris et al. (2008) Single muscle fiber adaptations to resistance training in old (>80 yr) men: evidence for limited skeletal muscle plasticity. Am J Physiol Regul Integr Comp Physiol 295:R273-80
Luden, Nicholas; Minchev, Kiril; Hayes, Erik et al. (2008) Human vastus lateralis and soleus muscles display divergent cellular contractile properties. Am J Physiol Regul Integr Comp Physiol 295:R1593-8
Raue, Ulrika; Slivka, Dustin; Jemiolo, Bozena et al. (2007) Proteolytic gene expression differs at rest and after resistance exercise between young and old women. J Gerontol A Biol Sci Med Sci 62:1407-12
Louis, Emily; Raue, Ulrika; Yang, Yifan et al. (2007) Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle. J Appl Physiol 103:1744-51
Raue, Ulrika; Slivka, Dustin; Jemiolo, Bozena et al. (2006) Myogenic gene expression at rest and after a bout of resistance exercise in young (18-30 yr) and old (80-89 yr) women. J Appl Physiol 101:53-9
Yang, Yifan; Jemiolo, Bozena; Trappe, Scott (2006) Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. J Appl Physiol 101:1442-50
Gallagher, P; Trappe, S; Harber, M et al. (2005) Effects of 84-days of bedrest and resistance training on single muscle fibre myosin heavy chain distribution in human vastus lateralis and soleus muscles. Acta Physiol Scand 185:61-9

Showing the most recent 10 out of 16 publications