Amyloid fibrils are associated with numerous debilitating diseases such as Alzheimer's disease, Parkinson's disease, Huntington's diseases, prion disease, familial amyloid polyneuropathy, senile systemic amyloidosis and type II diabetes, etc. The rational design of successful therapeutic strategies calls for detailed structural characterization of amyloid fibrils. Solid-state NMR and X-ray microcrystallography applied to short peptide fragments of amyloidogenic proteins have contributed greatly to our knowledge about the fibril core structure. The main goal of this project is to extend our understanding of the amyloid fibril structure by utilizing deep ultraviolet resonance Raman spectroscopy combined with hydrogen-deuterium exchange. This new method, recently pioneered in our laboratory, does not require isotope labeling and creates the opportunity for bridging the gap between our extended knowledge in the structural variations of model short peptide microcrystals and the core structure of amyloid fibrils prepared from entire proteins. We will determine and classify the core structure of amyloid fibrils prepared from full-length proteins. It has been postulated that differences in infectivity of prion protein aggregates, and related differences in the apparent symptoms of Creutzfeldt-Jakob disease patients, are possible due to amyloid polymorphism. Small molecules could potentially manipulate aggregate morphology and provide the basis for therapeutic approaches to suppress the formation of toxic aggregates. Therefore, it is critical to (i) have a robust method for structural characterization of polymorphs, (ii) understand the nature of amyloid polymorphs and their toxicity, and (iii) eventually, be able to control (suppress) the formation of toxic polymorphs. We will test alternative hypothesis concerning the level of structure at which functional polymorphisms are realized. When fully developed, in future studies, the proposed approach will make possible the comparative structural characterization of in vivo and in vitro amyloid fibrils. This will bridge the gap between in vivo and in vitro studies of neurodegenerative diseases. In particular, the method will provide unique information revealing the structural determinants of infectious and non-infectious amyloids.

Public Health Relevance

Successful outcomes of the proposed study will not only improve our understanding of amyloid fibril structure in general and the nature of amyloid polymorphs and their toxicity in particular, but also provide the basis for the development of therapeutic approaches to suppress the formation of toxic aggregates. When fully developed, the proposed approach will make possible the comparative structural characterization of in vivo and in vitro amyloid fibrils and bridge the gap between in vivo and in vitro studies of neurodegenerative diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG033719-04
Application #
8447473
Study Section
Macromolecular Structure and Function B Study Section (MSFB)
Program Officer
Wise, Bradley C
Project Start
2010-04-15
Project End
2015-03-31
Budget Start
2013-05-15
Budget End
2014-03-31
Support Year
4
Fiscal Year
2013
Total Cost
$192,116
Indirect Cost
$60,181
Name
State University of New York at Albany
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
152652822
City
Albany
State
NY
Country
United States
Zip Code
12222
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Sereda, Valentin; Ralbovsky, Nicole M; Vasudev, Milana C et al. (2016) Polarized Raman Spectroscopy for Determining the Orientation of di-D-phenylalanine Molecules in a Nanotube. J Raman Spectrosc 47:1056-1062
Sereda, Valentin; Sawaya, Michael R; Lednev, Igor K (2015) Structural Organization of Insulin Fibrils Based on Polarized Raman Spectroscopy: Evaluation of Existing Models. J Am Chem Soc 137:11312-20
Kurouski, Dmitry; Handen, Joseph D; Dukor, Rina K et al. (2015) Supramolecular chirality in peptide microcrystals. Chem Commun (Camb) 51:89-92
Ryzhikova, Elena; Kazakov, Oleksandr; Halamkova, Lenka et al. (2015) Raman spectroscopy of blood serum for Alzheimer's disease diagnostics: specificity relative to other types of dementia. J Biophotonics 8:584-96
Kurouski, Dmitry; Deckert-Gaudig, Tanja; Deckert, Volker et al. (2014) Surface characterization of insulin protofilaments and fibril polymorphs using tip-enhanced Raman spectroscopy (TERS). Biophys J 106:263-71
Sereda, Valentin; Lednev, Igor K (2014) Polarized Raman Spectroscopy of Aligned Insulin Fibrils. J Raman Spectrosc 45:665-671
Kurouski, Dmitry; Lu, Xuefang; Popova, Ludmila et al. (2014) Is supramolecular filament chirality the underlying cause of major morphology differences in amyloid fibrils? J Am Chem Soc 136:2302-12
Lednev, Igor K (2014) Amyloid fibrils: the eighth wonder of the world in protein folding and aggregation. Biophys J 106:1433-5
Bhat, Vikas; Olenick, Max B; Schuchardt, Brett J et al. (2013) Heat-induced fibrillation of BclXL apoptotic repressor. Biophys Chem 179:12-25

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