Rotifers are a potentially valuable new model to investigate the mechanisms of aging, as well as homeostasis and tissue regeneration, the effects of reproductive mode (asexual versus sexual) on longevity, and the cellular and molecular processes of senescence. Moreover, as basal metazoans, rotifers may share more genes with humans than Ecdyzoan animal models like C. elegans and D. melanogaster. In the proposed work we identify new genes associated with rotifer aging and search for their homologs in humans. Monogonont rotifers have specific features that make them an attractive models for aging studies, including: 1) a history of aging related research extending back nearly a century;2) asexual propagation of clonal cultures, so that experiments can take place in the same genetic background, without the potential inbreeding depression imposed on isogenic lines;3) sexual and asexual reproduction in the same genetic background;4) haploid males, allowing direct expression of alleles and simplifying crosses in the absence of complex marker chromosomes;5) production of highly stable diapausing eggs;5) many closely related strains and species that differ in life history traits;and 6) A well developed tool box of genetic resources including partially sequenced genomes and transcriptomes, and a working RNAi protocol. In the proposed work, we determine the effects on aging and longevity of dietary restriction, oxidative stress, and elevated temperature in five closely related strains of the Brachionus plicatilis species complex. The effect of sexual and asexual reproduction on aging also is investigated and correlated with cumulative oxidative damage to proteins, lipids, and DNA. We also identify genes involved in aging and longevity by examining the transcription profiles of rotifers of different ages under different dietary, reproductive, and stress conditions, and confirm the activity of specific genes by RNAi and qtPCR. The role of diapause in aging also is investigated by examining the mechanisms by which diapause in Brachionus alters tissue homeostasis, suspends metabolism during dormancy, and stimulates tissue repair following dormancy. The degree of aging observed in rotifers that have been kept in a dormant state for up to 25 years also is examined.

Public Health Relevance

Rotifers are a valuable new invertebrate model to investigate the mechanisms of aging, including homeostasis and tissue regeneration, the effects of reproductive mode (asexual versus sexual) and diapause on longevity, and the cellular and molecular processes of senescence. Because of their phylogenetic relationship to humans and other animals, they can be useful in the identification of genes that regulate aging. Identifying evolutionarily conserved genes in Ecdysozoan and spiralian phyla that regulate organismal aging could provide attractive targets for pharmacological intervention in mammalian aging. Molecular understanding of how dietary restriction extends lifespan will have direct implications for new approaches to treat metabolic diseases like diabetes and obesity.

National Institute of Health (NIH)
National Institute on Aging (NIA)
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Special Emphasis Panel (ZAG1-ZIJ-5 (M1))
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Murthy, Mahadev
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Georgia Institute of Technology
Schools of Arts and Sciences
United States
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Snell, Terry W; Johnston, Rachel K; Matthews, Amelia B et al. (2018) Repurposed FDA-approved drugs targeting genes influencing aging can extend lifespan and healthspan in rotifers. Biogerontology 19:145-157
Jones, Brande L; Walker, Chris; Azizi, Bahareh et al. (2017) Conservation of estrogen receptor function in invertebrate reproduction. BMC Evol Biol 17:65
Gribble, Kristin E; Mark Welch, David B (2017) Genome-wide transcriptomics of aging in the rotifer Brachionus manjavacas, an emerging model system. BMC Genomics 18:217
Snell, Terry W; Johnston, Rachel K; Srinivasan, Bharath et al. (2016) Repurposing FDA-approved drugs for anti-aging therapies. Biogerontology 17:907-920
Johnston, Rachel K; Snell, Terry W (2016) Moderately lower temperatures greatly extend the lifespan of Brachionus manjavacas (Rotifera): Thermodynamics or gene regulation? Exp Gerontol 78:12-22
Snell, Terry W; Johnston, Rachel K; Gribble, Kristin E et al. (2015) Rotifers as experimental tools for investigating aging. Invertebr Reprod Dev 59:5-10
Gribble, Kristin E; Jarvis, George; Bock, Martha et al. (2014) Maternal caloric restriction partially rescues the deleterious effects of advanced maternal age on offspring. Aging Cell 13:623-30
Snell, Terry W (2014) Rotifers as models for the biology of aging. Int Rev Hydrobiol 99:84-95
Smith, Hilary A; Snell, Terry W (2014) Differential evolution of asexual and sexual females in a benign culture environment. Int Rev Hydrobiol 99:117-124
Snell, Terry W; Johnston, Rachel K (2014) Glycerol extends lifespan of Brachionus manjavacas (Rotifera) and protects against stressors. Exp Gerontol 57:47-56

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