The long-term goal of our proposed research is to understand molecular basis of mitochondrial dysfunction Alzheimer's disease (AD) in pathogenesis and to develop neuroprotective strategies to delay or prevent the onset of AD. Increasing evidence suggests that amyloid beta (Ab), hyperphosphorylated tau and mitochondrial structural and functional abnormalities are critically involved in the loss of synapses and cognitive decline, in patients with Alzheimer's disease (AD). Several lines of evidence suggests that Ab and hyperphosphorylated tau are directly responsible for causing mitochondrial dysfunction and oxidative stress in AD pathogenesis. 1) Several studies found Ab and N-terminal tau in mitochondrial membranes and causing mitochondrial dysfunction in neurons affected by AD;2) recent studies found increased mRNA and protein levels of the mitochondrial fission genes and decreased fusion genes in AD postmortem and transgenic mouse models and cell-lines that express Ab, causing abnormal mitochondrial dynamics;3) several other studies found that Ab reduces total motile mitochondria, impairs mitochondrial axonal transport, particularly anterograde transport;inhibits synaptic ATP production;and causes synaptic degeneration in AD neurons and 4) further, GTPase protein, Drp1 interacted with Ab and hyperphosphorylated tau in neurons from AD patients and transgenic mouse models of Ab and tau. These findings lead to the hypothesis that the interaction of Drp1 with Ab and hyperphosphorylated tau triggers mitochondrial fission by enhancing Drp1 enzymatic activity and causes excessive mitochondrial fragmentation, and ultimate neuronal dysfunction selectively in AD neurons. The objectives of our application are 1) to determine whether Drp1 interactions with Ab and hyperphosphorylated tau increases with disease progression and pathogenesis;2) further how such interaction affects Drp1 enzymatic activity and mitochondrial morphology, distribution and function in AD neurons;3) in addition, whether partial loss of Drp1 decreases Ab and hyperphosphorylated tau-induced mitochondrial fragmentation, neuronal damage and synaptic dysfunction. The outcome of the proposed experiments in this application, will provide new insights in understanding the physiological relevance of interactions Drp1 with Ab, and phosphorylated tau in AD progression and pathogenesis and the outcome may have implications to develop mitochondrial therapeutics to reduce Ab and hyperphosphorylated tau-induced pathologies in AD patients.

Public Health Relevance

Mitochondrial dysfunction is a major hallmark of Alzheimer's disease (AD). Mitochondria play an important role in neurons and maintain the balance of mitochondrial fission and fusion. The objective of the proposed research is to determine whether Drp1 interactions with amyloid beta and with hyperphosphorylated tau affect Drp1 enzymatic activity and alter mitochondrial morphology, distribution, and function in AD neurons, and whether partial loss of Drp1 decreases amyloid beta and hyperphosphorylated tau-induced mitochondrial fragmentation, neuronal damage, and synaptic dysfunction.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
1R01AG042178-01A1
Application #
8451085
Study Section
Neural Oxidative Metabolism and Death Study Section (NOMD)
Program Officer
Refolo, Lorenzo
Project Start
2012-09-30
Project End
2017-04-30
Budget Start
2012-09-30
Budget End
2013-04-30
Support Year
1
Fiscal Year
2012
Total Cost
$439,653
Indirect Cost
$188,423
Name
Oregon Health and Science University
Department
Other Basic Sciences
Type
Other Domestic Higher Education
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
Reddy, P Hemachandra; Yin, XiangLing; Manczak, Maria et al. (2018) Mutant APP and amyloid beta-induced defective autophagy, mitophagy, mitochondrial structural and functional changes and synaptic damage in hippocampal neurons from Alzheimer's disease. Hum Mol Genet 27:2502-2516
Vijayan, Murali; Kumar, Subodh; Yin, Xiangling et al. (2018) Identification of novel circulatory microRNA signatures linked to patients with ischemic stroke. Hum Mol Genet 27:2318-2329
Reddy, P Hemachandra; Manczak, Maria; Yin, Xiangling et al. (2018) Protective Effects of Indian Spice Curcumin Against Amyloid-? in Alzheimer's Disease. J Alzheimers Dis 61:843-866
Kumar, Subodh; Reddy, P Hemachandra (2018) MicroRNA-455-3p as a Potential Biomarker for Alzheimer's Disease: An Update. Front Aging Neurosci 10:41
Reddy, P Hemachandra; Manczak, Maria; Yin, XiangLing et al. (2018) Synergistic Protective Effects of Mitochondrial Division Inhibitor 1 and Mitochondria-Targeted Small Peptide SS31 in Alzheimer's Disease. J Alzheimers Dis 62:1549-1565
Pradeepkiran, Jangampalli Adi; Reddy, Arubala P; Reddy, P Hemachandra (2018) Pharmacophore-based models for therapeutic drugs against phosphorylated tau in Alzheimer's disease. Drug Discov Today :
Kandimalla, Ramesh; Manczak, Maria; Yin, Xiangling et al. (2018) Hippocampal phosphorylated tau induced cognitive decline, dendritic spine loss and mitochondrial abnormalities in a mouse model of Alzheimer's disease. Hum Mol Genet 27:30-40
Reddy, A P; Reddy, P H (2017) Mitochondria-Targeted Molecules as Potential Drugs to Treat Patients With Alzheimer's Disease. Prog Mol Biol Transl Sci 146:173-201
Reddy, P Hemachandra; Manczak, Maria; Kandimalla, Ramesh (2017) Mitochondria-targeted small molecule SS31: a potential candidate for the treatment of Alzheimer's disease. Hum Mol Genet 26:1483-1496
Boles, Annette; Kandimalla, Ramesh; Reddy, P Hemachandra (2017) Dynamics of diabetes and obesity: Epidemiological perspective. Biochim Biophys Acta Mol Basis Dis 1863:1026-1036

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