The risk of most cancers, including leukemias, increases exponentially as we age, with over 90% of cancers occurring after the age of 50. This association has been primarily ascribed to the gradual accumulation of oncogenic mutations throughout life. We contend that the contribution of mutations, while necessary, is not sufficient to explain the role of aging in the development of leukemias and other cancers. Just as species evolution has been driven by environmental changes that select for adaptive phenotypes in populations, we propose that the changes in our tissues occurring in old age are substantial contributors to oncogenesis. In particular, inflammation increases in the bone marrow of the elderly, which contributes to impaired hematopoiesis. Our central hypothesis is that aging-dependent increases in inflammation are critical for enhancing selection for oncogenic mutations, and that dampening inflammation can reduce the risk of the associated leukemias. We previously have used mouse models to show that the aged and inflammatory bone marrow microenvironment reduces the fitness of B-cell progenitors, promoting selection for particular adaptive oncogenic events, leading to increased leukemogenesis. Here, we will explore how microenvironmental alterations in old age promote oncogenesis in immature hematopoietic stem and early progenitor cell (HSPC) pools at the apex of the hematopoietic hierarchy. We propose that C/EBP? and Myc activities are key hubs for oncogenic selection in aged bone marrow microenvironments due to their critical roles in balancing HSPC differentiation and self-renewal. Thus, we will develop interventions to reduce microenvironmental perturbations that deregulate C/EBP? and Myc and lead to oncogenesis in old age. To test our hypothesis, we will pursue two aims: 1) Determine whether aging and inflammation drive oncogenic adaptation in the HSPC compartment and 2) Identify mechanisms underlying increased oncogenesis with aging in HSPC pools. By determining whether and how microenvironmental changes impact HSPC fitness and thus oncogenic adaptation in old age, these results could provide a new explanation for links between aging and leukemia risk. In all, our proposed studies could provide answers for fundamental questions: Why do we get more leukemias as we age? Why are particular oncogenic mutations selected for in the bone marrow of the elderly? Can we alter aging-associated positive selection for oncogenic events and thus reduce leukemia risk? These studies could also identify interventions that reduce the risk of hematopoietic malignancies of old age by manipulating inflammatory factors that promote oncogenesis in the bone marrow microenvironment.

Public Health Relevance

Aging is highly associated with increased leukemia risk. A better understanding of how aging-associated inflammatory changes in the bone marrow microenvironment could promote leukemogenesis will be critical for the development of new prevention and treatment interventions that restore a healthy tissue landscape. While we may not be able to prevent the accumulation of oncogenic mutations in blood-forming hematopoietic stem and progenitor cells over a lifetime, modulating tissue microenvironments to reduce selection for oncogenically mutated cells could significantly reduce leukemia risk and improve health-span of our aging population.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Research Project (R01)
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Tumor Microenvironment Study Section (TME)
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Kerr, Candace L
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University of Colorado Denver
Schools of Medicine
United States
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