The objective of this research proposal is the elucidation of the structure and mechanism of synthesis of small, RNA-containing viruses with the expectation that many of the important results will be relevant to viruses in general and will contribute to an understanding of the interaction of viruses with their host cells. We will use standard methods such as centrifugation, electrophoresis, radioactive labeling, in vitro nucleic acid and protein synthesis and sequencing of proteins and nucleic acids. We will emphasize work with viruses whose genome is messenger RNA, in particular, the viruses Q beta, BMV, Nodamura, SBMV and their relatives.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI001466-29
Application #
3124081
Study Section
Virology Study Section (VR)
Project Start
1977-03-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
29
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Graduate Schools
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Shaklee, P N; Miglietta, J J; Palmenberg, A C et al. (1988) Infectious positive- and negative-strand transcript RNAs from bacteriophage Q beta cDNA clones. Virology 163:209-13