Abstract

Three major areas of study are projected in this research program. a) The molecular mechanisms of cell motility and chemotaxis will be further explored, with particular attention paid to the insertion of new membrane mass into the leading edge of a cell as a critical feature of cell extension. In addition, monoclonal antibodies directed to key cell surface components involved in chemotaxis and cell motility will be sought for. b) The molecular mechanisms involved in capping and related cell surface - cytoskeletal interactions will be investigated, focusing on a monoclonal antibody we have produced that appears to be directed to an integral protein that is associated with actin microfilaments underlying the cell membrane. c) The intracellular events involved in cytoxicity by natural killer cells and cytotoxic T lymphocytes (CTL) will be further explored in a system involving restricted recognition of CTL of target cells infected with lymphocytic choriomeningitis virus (LCMV), with which the nature of cell surface signalling events from the target cell to the effector cell will be explored.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI006659-23
Application #
3124299
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1975-05-01
Project End
1990-04-30
Budget Start
1987-05-01
Budget End
1988-04-30
Support Year
23
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Dutton, A H; Adams, M; Singer, S J (1990) Double fluorescence immunolabeling studies. Methods Enzymol 184:343-53
Kupfer, A; Burn, P; Singer, S J (1990) The PMA-induced specific association of LFA-1 and talin in intact cloned T helper cells. J Mol Cell Immunol 4:317-25
Kupfer, A; Singer, S J (1989) The specific interaction of helper T cells and antigen-presenting B cells. IV. Membrane and cytoskeletal reorganizations in the bound T cell as a function of antigen dose. J Exp Med 170:1697-713
Maher, P A (1989) Role of protein tyrosine phosphorylation in the NGF response. J Neurosci Res 24:29-37
Maher, P A; Pasquale, E B (1989) Heat shock induces protein tyrosine phosphorylation in cultured cells. J Cell Biol 108:2029-35
Kupfer, A; Singer, S J (1989) Cell biology of cytotoxic and helper T cell functions: immunofluorescence microscopic studies of single cells and cell couples. Annu Rev Immunol 7:309-37
Maher, P A; Pasquale, E B (1988) Tyrosine phosphorylated proteins in different tissues during chick embryo development. J Cell Biol 106:1747-55
Kupfer, A; Singer, S J (1988) Molecular dynamics in the membranes of helper T cells. Proc Natl Acad Sci U S A 85:8216-20
Pasquale, E B; Maher, P A; Singer, S J (1988) Comparative study of the tyrosine phosphorylation of proteins in Swiss 3T3 fibroblasts stimulated by a variety of mitogenic agents. J Cell Physiol 137:146-56
Kupfer, A; Kronebusch, P J; Rose, J K et al. (1987) A critical role for the polarization of membrane recycling in cell motility. Cell Motil Cytoskeleton 8:182-9

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