Abstract

1. To break the transmission chain of toxoplasmosis. 2. To mitigate the disease consequences of Toxoplasma infection by vaccination. 3. To develop genetically modified Toxoplasma and study infection and immunity produced by them in cats and non-feline modes. 4. To develop vaccines suitable for cats and non-feline mammals. 5. To study intestinal immunity in cats. 6. To further characterize the oocyst-induced cycle in cats. 7. To further characterize cycles, infection and immunity of other heteroxenous coccidia forming tissue cysts. The nature of immunity in cats will be studied after oral and parenteral infection and challenge by mouth. Some of the strains collected will be used as well as mutants of several types. Other mutants are to be developed, particularly those forming no oocysts or defective oocysts. For non-felines, mutants will be selected that show little central nervous tissue affinity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI007489-20
Application #
3124374
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1976-08-01
Project End
1988-03-31
Budget Start
1986-08-01
Budget End
1988-03-31
Support Year
20
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Kansas
Department
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Escajadillo, A; Frenkel, J K (1991) Experimental toxoplasmosis and vaccine tests in Aotus monkeys. Am J Trop Med Hyg 44:382-9
Frenkel, J K; Pfefferkorn, E R; Smith, D D et al. (1991) Prospective vaccine prepared from a new mutant of Toxoplasma gondii for use in cats. Am J Vet Res 52:759-63
Freyre, A; Dubey, J P; Smith, D D et al. (1989) Oocyst-induced Toxoplasma gondii infections in cats. J Parasitol 75:750-5
Sousa, O E; Saenz, R E; Frenkel, J K (1988) Toxoplasmosis in Panama: a 10-year study. Am J Trop Med Hyg 38:315-22
McLeod, R; Frenkel, J K; Estes, R G et al. (1988) Subcutaneous and intestinal vaccination with tachyzoites of Toxoplasma gondii and acquisition of immunity to peroral and congenital toxoplasma challenge. J Immunol 140:1632-7
Frenkel, J K; Escajadillo, A (1987) Cyst rupture as a pathogenic mechanism of toxoplasmic encephalitis. Am J Trop Med Hyg 36:517-22
Reyes, L; Frenkel, J K (1987) Specific and nonspecific mediation of protective immunity to Toxoplasma gondii. Infect Immun 55:856-63
Frenkel, J K (1985) Immunity in toxoplasmosis. Bull Pan Am Health Organ 19:354-67
Chinchilla, M; Frenkel, J K (1985) [Immunosuppressive activity of corticosteroids in primary infection and after the development of immunity against Toxoplasma and herpes virus] Rev Latinoam Microbiol 27:115-21