The long range goal of this proposal is to study the cell biology and biochemistry involved in the function of B cells as antigen presenting cells. We will focus our attention on two membrane glycoproteins that are known to be involved in this function, namely membrane immunogloblin and Ia-antigens. Furthermore we will attempt to gain information as to why activation of B cells seems to be important in allowing this cell to serve as an antigen presenting cell. As part of this investigation the mechanism by which Gamma-irradiation inhibits the antigen presenting cell capacity of these cells will be explored. We will also try and define other membrane glycoproteins or elements that might be involved in the antigen presenting capacity of B cells and in particular to evaluate the potential role of membrane proteases in the processing of certain antigens. Among the techniques that we plan to use to evaluate the role of Ig and membrane Iy in the antigen presenting capacity of B cells, we will purify these membrane glycoproteins and incorporate them into liposomes. These liposomes will either be utilized directly as antigen presenting units or they will be used as vehicles to incorporate these membrane molecules into other cell types in order to probe how they function in different cellular milieu.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
Project #
Application #
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Cytel Corporation
San Diego
United States
Zip Code
Sette, A; Sidney, J; Albertson, M et al. (1990) A novel approach to the generation of high affinity class II-binding peptides. J Immunol 145:1809-13
Sette, A; Buus, S; Appella, E et al. (1990) Structural requirements for the interaction between class II MHC molecules and peptide antigens. Immunol Res 9:2-7
Demotz, S; Grey, H M; Sette, A (1990) The minimal number of class II MHC-antigen complexes needed for T cell activation. Science 249:1028-30
Buus, S; Sette, A; Colon, S M et al. (1988) Autologous peptides constitutively occupy the antigen binding site on Ia. Science 242:1045-7
Krieger, J; Jenis, D M; Chesnut, R W et al. (1988) Studies on the capacity of intact cells and purified Ia from different B cell sources to function in antigen presentation to T cells. J Immunol 140:388-94
Grammer, S F; Ishioka, G Y; Chesnut, R W (1988) Studies on the capacity of B cells as well as T cells to serve as accessory cells for the activation of herpes simplex virus-specific cytolytic T cells. J Immunol 140:2016-22
Bekoff, M C; Cole, B C; Grey, H M (1987) Studies on the mechanism of stimulation of T cells by the Mycoplasma arthritidis-derived mitogen. Role of class II IE molecules. J Immunol 139:3189-94
Gay, D; Coeshott, C; Golde, W et al. (1986) The major histocompatibility complex-restricted antigen receptor on T cells. IX. Role of accessory molecules in recognition of antigen plus isolated IA. J Immunol 136:2026-32
Chesnut, R W; Grey, H M (1986) Antigen presentation by B cells and its significance in T-B interactions. Adv Immunol 39:51-94
Krieger, J I; Chesnut, R W; Grey, H M (1986) Capacity of B cells to function as stimulators of a primary mixed leukocyte reaction. J Immunol 137:3117-23

Showing the most recent 10 out of 15 publications