The objectives of the proposed study is to exploit the technology of immunoelectron microscopy to study various subcellular organelles and their function. In addition, the applicant proposes to study the plasmodial heat shock proteins and ookinete invasion of the mosquito midgut. The morphological changes of desferrioximine on the growth and development of malaria parasites will also be investigated. The pursuit of these studies will require the refinement and/or development of suitable immunoelectron microscopic techniques to prepare plasmodial preparations which retain antigenicity and ultrastructure. Such technology is required to study the subcellular structure and function of parasite specific organelles, as well as the subcellular localization of various plasmodial proteins such as the heat shock protein hsp 70 and glucose regulated proteins (grp 75.) In addition the effect of desferrioximine on in vitro and in vivo derived parasites will be determined to ascertain its usefulness in the treatment of cerebral malaria. The studies are aimed at providing a better understanding of the host parasite interaction and the subcellular level.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI010645-21
Application #
3124763
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1978-09-29
Project End
1994-07-31
Budget Start
1992-09-01
Budget End
1993-07-31
Support Year
21
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106