To analyze the possible H-2 restriction of the sensitization phase of contact hypersensitivity, murine skin will be painted with DNFB and grafted onto syngeneic and allogeneic hosts. Five days later, these recipients will be ear-challenged with DNFB. The role of graft adaptation in neonatal tolerance of H-2 transplantation alloantigens will be studied to determine whether this mechanism is operational in the long-term acceptance of H-2 disparate skin grafts. We have shown that long-term primed responder murine cell lines specifically recognize xenogeneic hamster determinants. We plan to analyze the fine specificity of this proliferative response, using T-cell clones generated by the limiting dilution method. The humoral and cellular immune responses of female rats have been assessed following pregnancy. With increasing parity, rejection of paternal allografts is increasingly impaired. This hyporesponsiveness is specific for paternal antigens, influenced by exposure to sperm and trophoblast, and arises at day 12 of pregnancy. Further investigation will define the role of fetal cells escaping across the placenta and the uterine exposure site and the extensive maternal decidua that develops. It has been suggested that the reproductive efficiency of MHC-compatible pregnancies is less than that of MHC-incompatible pregnancies. To test this hypothesis, we will use the following parameters in rats to compare the efficiency of MHC-compatible (DA-female times ACI-male, ACI-female times DA-male) and MHC-incompatible (DA-female times FI-male, ACI-female times-FI male) pregnancies: 1) percentage of animals that mate; 2) number of pregnancies resulting; and 3) litter size, number born dead.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI010678-14
Application #
3124765
Study Section
Immunobiology Study Section (IMB)
Project Start
1977-07-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Type
Overall Medical
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390
Zuckermann, F A; Head, J R (1988) Murine trophoblast resists cell-mediated lysis. II. Resistance to natural cell-mediated cytotoxicity. Cell Immunol 116:274-86
Head, J R; Drake, B L; Zuckermann, F A (1987) Major histocompatibility antigens on trophoblast and their regulation: implications in the maternal-fetal relationship. Am J Reprod Immunol Microbiol 15:12-8
Head, J R; Miller, S T; Kresge, C K (1987) Dendritic accessory cells in the pregnant and nonpregnant rat uterus. Transplant Proc 19:565-6
Zuckermann, F A; Head, J R (1987) Murine trophoblast resists cell-mediated lysis. I. Resistance to allospecific cytotoxic T lymphocytes. J Immunol 139:2856-64
Zuckermann, F A; Head, J R (1987) Possible mechanism of non-rejection of the feto-placental allograft: trophoblast resistance to lysis by cellular immune effectors. Transplant Proc 19:554-6
Seelig Jr, L L; Head, J R (1987) Uptake of lymphocytes fed to suckling rats. An autoradiographic study of the transit of labeled cells through the neonatal gastric mucosa. J Reprod Immunol 10:285-97
Head, J R; Billingham, R E (1986) Concerning the immunology of the uterus. Am J Reprod Immunol Microbiol 10:76-81
Zuckermann, F A; Head, J R (1986) Isolation and characterization of trophoblast from murine placenta. Placenta 7:349-64
Head, J R; Gaede, S D (1986) Ia antigen expression in the rat uterus. J Reprod Immunol 9:137-53
Takashima, A; Billingham, R E; Grinnell, F (1986) Activation of rabbit keratinocyte fibronectin receptor function in vivo during wound healing. J Invest Dermatol 86:585-90

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