Abstract

The objective of these studies is to determine the molecular and cellular interactions that lead to the selective expression of IgA in secretory glands and to the selective transport of IgA into external secretions. The structural basis of the binding of immunoglobulins to specific receptors on epithelial cells will be investigated and we shall attempt to identify and characterize the immun globulin binding receptor. Properties of unstimulated and mitogen-stimulated colostral lymphocytes will be determined. With the use of immunofluorescence techniques, we shall determine the intracellular and membrane-associated immunogl bulin class. The presence of receptors for secretory component will be determined by immunofluorescence on the membrane and in cytoplasm of stimulated and unstimulated lymphocytes. The effect of co-cultivation of peripheral bl od or col stral lymphocytes and normal or tum r epithelial cells on the expression of the immunoglobulin isotype will be studied. With the use of physicochemical techniques, conformational studies will be made of constituent components of secretory IgA molecules.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI010854-14
Application #
3124831
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1978-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Renegar, Kathryn B; Menge, Alan; Mestecky, Jiri (2006) Influenza virus infection of the murine uterus: a new model for antiviral immunity in the female reproductive tract. Viral Immunol 19:613-22
Takahashi, T; Iwase, T; Takenouchi, N et al. (1996) The joining (J) chain is present in invertebrates that do not express immunoglobulins. Proc Natl Acad Sci U S A 93:1886-91
Crowley-Nowick, P A; Campbell, E; Schrohenloher, R E et al. (1996) Polyethylene glycol precipitates of serum contain a large proportion of uncomplexed immunoglobulins and C3. Immunol Invest 25:91-101
Crowley-Nowick, P A; Campbell, J E; Mullins, A L et al. (1995) Human IgA1 and IgA2 have distinct spectrotypes but display subclass similarities between individuals. Adv Exp Med Biol 371A:591-3
Crowley-Nowick, P A; Bull, R; van den Wall Bake, A W et al. (1994) Immunological studies of IgA nephropathy in blacks reveal elevations of serum IgA2 as well as IgA1. Nephrol Dial Transplant 9:1324-9
Endo, T; Mestecky, J; Kulhavy, R et al. (1994) Carbohydrate heterogeneity of human myeloma proteins of the IgA1 and IgA2 subclasses. Mol Immunol 31:1415-22
Iwase, T; Saito, I; Takahashi, T et al. (1993) Early expression of human J chain and mu chain gene in the fetal liver. Cell Struct Funct 18:297-302
Menge, A C; Mestecky, J (1993) Surface expression of secretory component and HLA class II DR antigen on glandular epithelial cells from human endometrium and two endometrial adenocarcinoma cell lines. J Clin Immunol 13:259-64
Tomana, M; Zikan, J; Moldoveanu, Z et al. (1993) Interactions of cell-surface galactosyltransferase with immunoglobulins. Mol Immunol 30:265-75
Mestecky, J (1993) Saliva as a manifestation of the common mucosal immune system. Ann N Y Acad Sci 694:184-94

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