The detailed mechanism of assembly of Tobacco Mosaic Virus and other viruses will be investigated by physical-chemical techniques. Specifically, the elementary step kinetics of the assembly of Tobacco Mosaic Virus will be studied using stopped-flow and temperature-jump relaxation kinetics and other physical methods. The assembly mechanisms of icosahedral and tubular viruses will be analyzed with the following question in mind: Are there general kinetic principles governing the assembly of these two structural classes of viruses? Specifically, the role of metastable protein aggregates in virus assembly will be studied; the chemical and structural basis for recognition of the TMV-RNA by the coat protein at the assembly nucleation site will be determined; the conformation switching in the 20S protein aggregate that occurs during assembly will be studied by time-resolved x-ray diffraction. These physical-chemical solution studies will be interpreted in terms of the atomic resolution structure of the virus that is beng determined elsewhere.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI011573-12
Application #
3124971
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1977-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
12
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Connecticut
Department
Type
Schools of Arts and Sciences
DUNS #
City
Storrs-Mansfield
State
CT
Country
United States
Zip Code
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