Abstract

The proposed work is designed to study the interactions between the products of antigen-stimulated immunocompetent cells and other cell types in various forms of cell-mediated immunity. Our current work will focus on murine models. We will investigate lymphokine production in abnormal strains such as the NZB/W mouse. In this animal there is diminished reactivity to DNA and other antigens with respect to delayed hypersensitivity. This hyporeactivity may be corrected with prostaglandins in vivo. We will determine whether the defect is at the level of MIF and/or chemotactic factor production. Additional experiments will focus on the detection of chemotactic events in vivo. The model to be used here involves the installation of antigen or performed mediator into the peritoneal cavity and determining the kinetics of the various types of inflammatory cells. In those studies involving production of mediators, we will pay special attention to the role of ancillary helping cells such as the macrophage. We will determine the relative capacity of such phagocytic cells from various sources to serve such a role and attempt to determine the mechanism by which such interaction occurs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
7R01AI012477-13
Application #
3125204
Study Section
(SSS)
Project Start
1987-06-01
Project End
1988-05-31
Budget Start
1987-06-01
Budget End
1988-05-31
Support Year
13
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Hahnemann University
Department
Type
Schools of Medicine
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19129

  Publications

Wong, R L; Clark, R B; Gutowski, J K et al. (1990) A cytoplasmic activator of DNA replication is involved in signal transduction in antigen-specific T cell lines. Cell Immunol 127:382-90
Munoz-Blay, T; Nieburgs, A C; Korn, J H et al. (1989) Inhibition of thymocyte mitogenic responses by coculture with thymic epithelial cells. Clin Immunol Immunopathol 51:275-93
Munoz-Blay, T; Rollo, A; Cone, R E et al. (1988) Characterization of the molecules involved in thymocyte thymic epithelial cell adhesion. Clin Immunol Immunopathol 48:343-53
Nieburgs, A C; Korn, J H; Picciano, P T et al. (1987) Thymic epithelium in vitro. IV. Regulation of growth and mediator production by epidermal growth factor. Cell Immunol 108:396-404
Wong, R L; Gutowski, J K; Katz, M et al. (1987) Induction of DNA synthesis in isolated nuclei by cytoplasmic factors: inhibition by protease inhibitors. Proc Natl Acad Sci U S A 84:241-5
Munoz-Blay, T; Nieburgs, A C; Cohen, S (1987) Thymic epithelium in vitro. V. Binding of thymocytes to cultured thymic epithelial cells. Cell Immunol 109:371-83
Nieburgs, A C; Picciano, P; Korn, J H et al. (1987) Thymic epithelium in vitro. III. Cytokine production by a thymic epithelial subset. Cell Immunol 104:182-9
Gutowski, J K; Cohen, S (1987) Suppression of DNA synthesis in normal, but not neoplastic, nuclei by cytoplasmic extracts from resting cells. Cell Immunol 106:174-9
Wong, R L; Katz, M E; Ogata, K et al. (1987) Inhibition of nuclear DNA synthesis by an autoantibody to proliferating cell nuclear antigen/cyclin. Cell Immunol 110:443-8
Cohen, S (1986) Physiologic and pathologic manifestations of lymphokine action. Hum Pathol 17:112-21

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