Abstract

We propose to continue and greatly extend our studies on the molecular biology of picornaviruses, particularly on poliovirus. We shall study aspects of viral protein synthesis (initiation and processing of polypeptides) and the intriguing problem of viral RNA replication. We will use recombinant DNA technology to define gene function (in vitro recombination, site specific mutagenesis) and we plan to characterize the surface properties of the virion (neutralizing epitopes, virus-cell attachment sites). Our studies may lead to the development of new vaccines and drugs directed against picornavirus infection.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI015122-14
Application #
3126019
Study Section
Experimental Virology Study Section (EVR)
Project Start
1978-04-01
Project End
1988-03-31
Budget Start
1987-04-01
Budget End
1988-03-31
Support Year
14
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Chen, Ying-Han; Du, WenLi; Hagemeijer, Marne C et al. (2015) Phosphatidylserine vesicles enable efficient en bloc transmission of enteroviruses. Cell 160:619-630
Paul, Aniko V; Wimmer, Eckard (2015) Initiation of protein-primed picornavirus RNA synthesis. Virus Res 206:12-26
Jiang, Ping; Liu, Ying; Ma, Hsin-Chieh et al. (2014) Picornavirus morphogenesis. Microbiol Mol Biol Rev 78:418-37
Ma, Hsin-Chieh; Liu, Ying; Wang, Chunling et al. (2014) An interaction between glutathione and the capsid is required for the morphogenesis of C-cluster enteroviruses. PLoS Pathog 10:e1004052
Schein, Catherine H; Bowen, David M; Lewis, Jessica A et al. (2012) Physicochemical property consensus sequences for functional analysis, design of multivalent antigens and targeted antivirals. BMC Bioinformatics 13 Suppl 13:S9
Wang, Chunling; Jiang, Ping; Sand, Claire et al. (2012) Alanine scanning of poliovirus 2CATPase reveals new genetic evidence that capsid protein/2CATPase interactions are essential for morphogenesis. J Virol 86:9964-75
Song, Yutong; Liu, Ying; Ward, Charles B et al. (2012) Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design. Proc Natl Acad Sci U S A 109:14301-7
Toyoda, Hidemi; Wimmer, Eckard; Cello, Jeronimo (2011) Oncolytic poliovirus therapy and immunization with poliovirus-infected cell lysate induces potent antitumor immunity against neuroblastoma in vivo. Int J Oncol 38:81-7
Wimmer, Eckard; Paul, Aniko V (2011) Synthetic poliovirus and other designer viruses: what have we learned from them? Annu Rev Microbiol 65:583-609
Mueller, Steffen; Coleman, J Robert; Papamichail, Dimitris et al. (2010) Live attenuated influenza virus vaccines by computer-aided rational design. Nat Biotechnol 28:723-6

Showing the most recent 10 out of 134 publications