Abstract

This is a grant application to continue our studies of antibodies to the simple antigenic determinants phosphocholine (PC), group A streptococcal carbohydrate (GAC) and alpha (1 yields 3) dextran. Our goal in these studies is to provide evidence of how antibody diversity is generated. In particular, in the present proposal we address questions about how diverse antibodies really are and whether evolutionary relationships are preserved among antibodies with similar specificity. In addition, we will examine questions concerning combinatorial diversity generated by heavy-light chain pairing and whether VH genes pair randomly with all CH genes. Finally, we will probe the relationship of VH region expression and L chain expression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI015926-07
Application #
3126484
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1979-08-01
Project End
1988-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Stanley Jr, S L; Foster, L; Phillips, N (1992) Molecular analysis of carbohydrate antigen-induced monoclonal IgM anti-IgG antibodies (rheumatoid factors). Mol Immunol 29:453-61
Stanley Jr, S L; Huizenga, H; Li, E (1992) Isolation and partial characterization of a surface glycoconjugate of Entamoeba histolytica. Mol Biochem Parasitol 50:127-38
Phillips, N J; Davie, J M (1990) Idiotope structure and genetic diversity in anti-streptococcal group A carbohydrate antibodies. J Immunol 145:915-24
Stanley Jr, S L; Li, E; Davie, J M (1988) Antigen induced rheumatoid factors: characteristics of monoclonal rheumatoid factors produced after protein and carbohydrate immunization. Mol Immunol 25:285-94
Lutz, C T; Davie, J M (1988) Genetics and primary structure of V kappa gene segments encoding antibody to streptococcal group A carbohydrate. Comparison of V kappa gene structure with idiotope expression. J Immunol 140:641-5
Starren, J B; Davie, J M (1987) Ir gene-controlled response to haptenated hen ovomucoid: isotypic specificity and dominant nonresponsiveness. Cell Immunol 104:59-70
Stanley Jr, S L; Bischoff, J K; Davie, J M (1987) Antigen-induced rheumatoid factors. Protein and carbohydrate antigens induce different rheumatoid factor responses. J Immunol 139:2936-41
Greenspan, N S; Monafo, W J; Davie, J M (1987) Interaction of IgG3 anti-streptococcal group A carbohydrate (GAC) antibody with streptococcal group A vaccine: enhancing and inhibiting effects of anti-GAC, anti-isotypic, and anti-idiotypic antibodies. J Immunol 138:285-92
Lutz, C T; Bartholow, T L; Greenspan, N S et al. (1987) Molecular dissection of the murine antibody response to streptococcal group A carbohydrate. J Exp Med 165:531-45
Monafo, W J; Greenspan, N S; Cebra-Thomas, J A et al. (1987) Modulation of the murine immune response to streptococcal group A carbohydrate by immunization with monoclonal anti-idiotope. J Immunol 139:2702-7

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