Abstract

The molecular basis of E. coli colonization of the large intestine will be investigated using a mouse model. Mutants of normal human fecal strains of E. coli, which have an impaired ability to colonize the large intestine as a result of limited genetic manipulation and which possess well-defined altered surface properties will be used to investigate microbial interaction with the mucous gel blanket of the large intestine. Bacterial binding of soluble mucous gel glycoproteins and bacterial adhesion to immobilized mucous gel glycoproteins will be assessed in vitro and compared to in vivo colonizing ability. The bacterial surface components (adhesins) and individual mucous gel components (receptors) involved in the interaction will be isolated and biochemically characterized. The overall objective of the proposed research is to characterize bacterial-mucous gel interactions and to clarify the role of the mucous gel in E. coli colonization of the large intestine.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI016370-06
Application #
3126642
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1980-09-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Rhode Island
Department
Type
Schools of Arts and Sciences
DUNS #
135531015
City
Kingston
State
RI
Country
United States
Zip Code

  Publications

Utley, M; Franklin, D P; Krogfelt, K A et al. (1998) A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice. FEMS Microbiol Lett 163:129-34
Newman, J V; Burghoff, R L; Pallesen, L et al. (1994) Stimulation of Escherichia coli F-18Col- type-1 fimbriae synthesis by leuX. FEMS Microbiol Lett 122:281-7
McCormick, B A; Klemm, P; Krogfelt, K A et al. (1993) Escherichia coli F-18 phase locked 'on' for expression of type 1 fimbriae is a poor colonizer of the streptomycin-treated mouse large intestine. Microb Pathog 14:33-43
Burghoff, R L; Pallesen, L; Krogfelt, K A et al. (1993) Utilization of the mouse large intestine to select an Escherichia coli F-18 DNA sequence that enhances colonizing ability and stimulates synthesis of type 1 fimbriae. Infect Immun 61:1293-300
Metcalfe, J W; Krogfelt, K A; Krivan, H C et al. (1991) Characterization and identification of a porcine small intestine mucus receptor for the K88ab fimbrial adhesin. Infect Immun 59:91-6
Krogfelt, K A; McCormick, B A; Burghoff, R L et al. (1991) Expression of Escherichia coli F-18 type 1 fimbriae in the streptomycin-treated mouse large intestine. Infect Immun 59:1567-8
McCormick, B A; Laux, D C; Cohen, P S (1990) Neither motility nor chemotaxis plays a role in the ability of Escherichia coli F-18 to colonize the streptomycin-treated mouse large intestine. Infect Immun 58:2957-61
Cohen, P S; Kjelleberg, S; Laux, D C et al. (1990) Escherichia coli F-18 makes a streptomycin-treated mouse large intestine colonization factor when grown in nutrient broth containing glucose. Infect Immun 58:1471-2
Conway, P L; Welin, A; Cohen, P S (1990) Presence of K88-specific receptors in porcine ileal mucus is age dependent. Infect Immun 58:3178-82
Burghoff, R L; Laux, D C; Cohen, P S (1990) Construction of stable cloning vectors that do not segregate from a human fecal Escherichia coli strain in the streptomycin-treated mouse large intestine. Infect Immun 58:1141-5

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