Abstract

The overall goal of this research is to elucidate mechanisms that regulate genetic expression at the levels of transcription and RNA processing and to understand the importance of these mechanisms for B lymphocyte development and ribosome biogenesis. Studies of immunoglobulin gene regulation in the mouse will include (i) analysis of the parameters governing the regulation of heavy chain mRNA production by alternative RNA processing, (ii) characterization of a novel V(kappa) gene promoter element and its cognate transcription factor, (iii) an attempt to define the roles of intronic and downstream enhancers for the establishment and maintenance of kappa gene transcription, and (iv) an exploration of immunoglobulin gene regulation in FACS-fractionated populations of B lymphoid cells. Studies of mouse ribosomal protein genes will include (i) characterization of the factors that recognize the distinctive promoters of these genes and cloning of the factor-encoding genes, (ii) elucidation of interactions between the various promoter elements, and (iii) a study of the importance of intron splicing for the stabilization and efficient intracellular transpose of mRNA.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI017330-15
Application #
2060494
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1981-01-01
Project End
1995-12-31
Budget Start
1995-01-01
Budget End
1995-12-31
Support Year
15
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
Institute for Cancer Research
Department
Type
DUNS #
872612445
City
Philadelphia
State
PA
Country
United States
Zip Code
19111

  Publications

Genuario, R R; Perry, R P (1996) The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J Biol Chem 271:4388-95
Stokes, D G; Perry, R P (1995) DNA-binding and chromatin localization properties of CHD1. Mol Cell Biol 15:2745-53
Safrany, G; Perry, R P (1995) The relative contributions of various transcription factors to the overall promoter strength of the mouse ribosomal protein L30 gene. Eur J Biochem 230:1066-72
Safrany, G; Perry, R P (1993) Transcription factor RFX1 helps control the promoter of the mouse ribosomal protein-encoding gene rpL30 by binding to its alpha element. Gene 132:279-83
Safrany, G; Perry, R P (1993) Characterization of the mouse gene that encodes the delta/YY1/NF-E1/UCRBP transcription factor. Proc Natl Acad Sci U S A 90:5559-63
Delmas, V; Stokes, D G; Perry, R P (1993) A mammalian DNA-binding protein that contains a chromodomain and an SNF2/SWI2-like helicase domain. Proc Natl Acad Sci U S A 90:2414-8
Genuario, R R; Kelley, D E; Perry, R P (1993) Comparative utilization of transcription factor GABP by the promoters of ribosomal protein genes rpL30 and rpL32. Gene Expr 3:279-88
Chung, S; Perry, R P (1993) The importance of downstream delta-factor binding elements for the activity of the rpL32 promoter. Nucleic Acids Res 21:3301-8
Chung, S; Perry, R P (1991) Cell-free transcription of a mouse ribosomal-protein-encoding gene: the effects of promoter mutations. Gene 100:173-80
Hariharan, N; Kelley, D E; Perry, R P (1991) Delta, a transcription factor that binds to downstream elements in several polymerase II promoters, is a functionally versatile zinc finger protein. Proc Natl Acad Sci U S A 88:9799-803

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