The T-even bacteriophage - E. coli system will be investigated as a model system of viral invasion, viral structure and viral assembly. Recent results have been identified a number of phage genes which produce a set of enzymes which ultimately form a unique type of folic acid - a dihydropteroyl hexaglutamate. In addition these enzymes have been found to be structural components of the central hub of the tail baseplate of the phage particle. Work will be devoted to determine: 1) The pathway or sequence of the phage gene products take to assemble the central baseplate hub; 2) The nature of the interaction of these hub components with each other during the formation of phage-induced folate; 3) Development of new methods to determine the assembly sequence pathway including the use of inhibitors to accumulate intermediates and selective labeling of different hub components. 4) The nature of the interaction of the hub components with host cell membrane. 5) The nature of the interaction of these hub components with the tail fibers especially the interaction of T4 gene product 28 with the zinc-containing short tail fiber. It is hoped that the detailed chemical knowledge gained from these studies will lead to the control of virus assembly and to new principles involving assembly of other viruses including those causing human diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI018370-09
Application #
3127867
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1981-02-01
Project End
1991-01-31
Budget Start
1989-02-01
Budget End
1991-01-31
Support Year
9
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Kozloff, L M; Lute, M; Arellano, F et al. (1992) Bacterial ice nucleation activity after T4 bacteriophage infection. J Gen Microbiol 138:941-4
Kozloff, L M; Turner, M A; Arellano, F et al. (1991) Phosphatidylinositol, a phospholipid of ice-nucleating bacteria. J Bacteriol 173:2053-60
Kozloff, L M; Turner, M A; Arellano, F (1991) Formation of bacterial membrane ice-nucleating lipoglycoprotein complexes. J Bacteriol 173:6528-36
Turner, M A; Arellano, F; Kozloff, L M (1991) Components of ice nucleation structures of bacteria. J Bacteriol 173:6515-27
Szewczyk, B; Kozloff, L M (1985) A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose. Anal Biochem 150:403-7