Abstract

Urinary tract infections (UTI) are among the most common human infections and may have serious sequelace. Most UTI are caused by members of the bacterial family Enterbacteriaceae. UTI results from the interaction of Bacteria with a susceptible host. Each step in the pathogenic process is determined by the balance between host. Each step in the pathogenic process id determined by the balance between host resistance and bacterial virulence. The virulent strain possesses determinants required to colonize the bowel, ascend into and persist in the urinary tract and induce infection. The object of this research is to characterize and compare, using modern genetic techniques, bacterial genes that encode virulence determinants and to demonstrate the role of these virulence genes in UT1. More specifically, a variety of methods will be used for the genetic, physical and functional analysis of receptor specific proteins that mediate adherence of Proteus mirabilis and Escherichia coli to human uroepithelium.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI018462-09
Application #
3127963
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Project Start
1982-01-01
Project End
1994-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
9
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Hull, S I; Hull, R A (1995) Molecular cloning of adhesion genes. Methods Enzymol 253:258-69
Cook, S W; Mody, N; Valle, J et al. (1995) Molecular cloning of Proteus mirabilis uroepithelial cell adherence (uca) genes. Infect Immun 63:2082-6
Hull, R A; Nowicki, B; Kaul, A et al. (1994) Effect of pap copy number and receptor specificity on virulence of fimbriated Escherichia coli in a murine urinary tract colonization model. Microb Pathog 17:79-86
Klann, A G; Hull, R A; Palzkill, T et al. (1994) Alanine-scanning mutagenesis reveals residues involved in binding of pap-3-encoded pili. J Bacteriol 176:2312-7
Bahrani, F K; Cook, S; Hull, R A et al. (1993) Proteus mirabilis fimbriae: N-terminal amino acid sequence of a major fimbrial subunit and nucleotide sequences of the genes from two strains. Infect Immun 61:884-91
Batchelor, R A; Alifano, P; Biffali, E et al. (1992) Nucleotide sequences of the genes regulating O-polysaccharide antigen chain length (rol) from Escherichia coli and Salmonella typhimurium: protein homology and functional complementation. J Bacteriol 174:5228-36
Klann, A G; Hull, R A; Hull, S I (1992) Sequences of the genes encoding the minor tip components of Pap-3 pili of Escherichia coli. Gene 119:95-100
Batchelor, R A; Haraguchi, G E; Hull, R A et al. (1991) Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli. J Bacteriol 173:5699-704
Haraguchi, G E; Zahringer, U; Jann, B et al. (1991) Genetic characterization of the O4 polysaccharide gene cluster from Escherichia coli. Microb Pathog 10:351-61
Andersson, P; Engberg, I; Lidin-Janson, G et al. (1991) Persistence of Escherichia coli bacteriuria is not determined by bacterial adherence. Infect Immun 59:2915-21

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