Abstract

This proposal focuses on the human respiratory pathogen, Mycoplasma pneumoniae. Emphasis is directed at analyzing mycoplasma binding sites (ligands, adhesins) responsible for surface parasitism of respiratory cells; purifying and characterizing host cell receptors that mediate this cytadsorption; generating monospecific and monoclonal antibodies against key M. pneumoniae membrane proteins; and examining membrane properties of mycoplasmas in order to assess topography and function of mycoplasma adhesins and accessory proteins. These experimental approaches require the use of radiolabeling techniques, acrylamide gel electrophoresis, column chromatography, basic microbiological-tissue culture procedures and handling of experimental animals. Also generation of monospecific and monoclonal antibodies will be necessary along with immunoferritin electron microscopy. This infectious disease model should provide fundamental information that clarifies host-parasite (membrane-membrane) interactions at biochemical-immunological-molecular levels. Effort is made to demonstrate the unique advantages of this system as an important biological resource in the study of human airway disease and in the analysis of structural-functional interactions among membrane macromolecules.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI018540-02
Application #
3128004
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
Overall Medical
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

Su, C J; Dallo, S F; Baseman, J B (1990) Molecular distinctions among clinical isolates of Mycoplasma pneumoniae. J Clin Microbiol 28:1538-40
Dallo, S F; Baseman, J B (1990) Cross-hybridization between the cytadhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium and genomic DNA of Mycoplasma gallisepticum. Microb Pathog 8:371-5
Dallo, S F; Horton, J R; Su, C J et al. (1990) Restriction fragment length polymorphism in the cytadhesin P1 gene of human clinical isolates of Mycoplasma pneumoniae. Infect Immun 58:2017-20
Su, C J; Chavoya, A; Baseman, J B (1989) Spontaneous mutation results in loss of the cytadhesin (P1) of Mycoplasma pneumoniae. Infect Immun 57:3237-9
Dallo, S F; Chavoya, A; Su, C J et al. (1989) DNA and protein sequence homologies between the adhesins of Mycoplasma genitalium and Mycoplasma pneumoniae. Infect Immun 57:1059-65
Dallo, S F; Horton, J R; Su, C J et al. (1989) Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium. Microb Pathog 6:69-73
Baseman, J B; Dallo, S F; Tully, J G et al. (1988) Isolation and characterization of Mycoplasma genitalium strains from the human respiratory tract. J Clin Microbiol 26:2266-9
Dallo, S F; Su, C J; Horton, J R et al. (1988) Identification of P1 gene domain containing epitope(s) mediating Mycoplasma pneumoniae cytoadherence. J Exp Med 167:718-23
Su, C J; Chavoya, A; Baseman, J B (1988) Regions of Mycoplasma pneumoniae cytadhesin P1 structural gene exist as multiple copies. Infect Immun 56:3157-61
Morrison-Plummer, J; Lazzell, A; Baseman, J B (1987) Shared epitopes between Mycoplasma pneumoniae major adhesin protein P1 and a 140-kilodalton protein of Mycoplasma genitalium. Infect Immun 55:49-56

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