Abstract

The cell surface receptor for IgE will be further characterized. Interaction of radiolabeled C3a with rat basopholic leukemia (RBL) cells and rat mast cells will be studied to determine if there is any interaction between IgE receptors and possible C3a receptors. The orientation of the IgE receptor in the plasma membrane will be examined especially to determine if any subcomponent of the receptor is exposed at the cytoplasmic side of the membrane. The receptor will be examined for other biologic activities, specifically radiolabeled disopropylfluorophosphate (DFP) will be used to tag the serine esterase that is activated in IgE-mediated reactions; the labeled esterase will be examined for possible identity with the receptor. The heterogeneity of Fc receptors on mast cells and on RBL cells will be further examined; the Fc receptor that mediates phagocytosis by RMC and RBL cells will be characterized and compared to the Fc receptor for IgE that mediates degranulation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI018697-04
Application #
3128113
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1981-05-01
Project End
1987-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Martin, Rebecca K; Damle, Sheela R; Valentine, Yolander A et al. (2018) B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade. Cell Rep 22:1824-1834
Damle, S R; Martin, R K; Cockburn, C L et al. (2018) ADAM10 and Notch1 on murine dendritic cells control the development of type 2 immunity and IgE production. Allergy 73:125-136
Damle, Sheela R; Martin, Rebecca K; Cross, Janet V et al. (2017) Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis. Mucosal Immunol 10:205-214
Lownik, Joseph C; Luker, Andrea J; Damle, Sheela R et al. (2017) ADAM10-Mediated ICOS Ligand Shedding on B Cells Is Necessary for Proper T Cell ICOS Regulation and T Follicular Helper Responses. J Immunol 199:2305-2315
Cooley, Lauren Folgosa; El Shikh, Mohey Eldin; Li, Wei et al. (2016) Impaired immunological synapse in sperm associated antigen 6 (SPAG6) deficient mice. Sci Rep 6:25840
Cooley, Lauren Folgosa; Martin, Rebecca K; Zellner, Hannah B et al. (2015) Increased B Cell ADAM10 in Allergic Patients and Th2 Prone Mice. PLoS One 10:e0124331
Martin, Rebecca K; Brooks, Keith B; Henningsson, Frida et al. (2014) Antigen transfer from exosomes to dendritic cells as an explanation for the immune enhancement seen by IgE immune complexes. PLoS One 9:e110609
Martin, Rebecca K; Saleem, Sheinei J; Folgosa, Lauren et al. (2014) Mast cell histamine promotes the immunoregulatory activity of myeloid-derived suppressor cells. J Leukoc Biol 96:151-9
Folgosa, Lauren; Zellner, Hannah B; El Shikh, Mohey Eldin et al. (2013) Disturbed follicular architecture in B cell A disintegrin and metalloproteinase (ADAM)10 knockouts is mediated by compensatory increases in ADAM17 and TNF-? shedding. J Immunol 191:5951-8
Schlosburg, Joel E; O'Neal, Scott T; Conrad, Daniel H et al. (2011) CB1 receptors mediate rimonabant-induced pruritic responses in mice: investigation of locus of action. Psychopharmacology (Berl) 216:323-31

Showing the most recent 10 out of 65 publications