Abstract

of antigenic epitopes on protein I and protein III. We have a large number of clones producing antibodies to PIB proteins and we have the sequence of a PIB gene nearly complete. Using deletion mutants and synthetic peptides the reactive eptiopes will be defined. It has recently been demonstrated that human antibodies to PIII are able to block bactericidal activity of antibodies directed to other surface antigens. We have completed the sequence of the structural gene of PIII and this has allowed the proposal of a model of the disposition of this protein in the outer membrane. We are currently isolating PIII reactive monoclonal antibodies and will determine the antigenic epitopes of PIII, their surface exposure, and which one generates the blocking antibodies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI019469-05
Application #
3128803
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1982-02-01
Project End
1988-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
5
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Apicella, M A; Ketterer, M; Lee, F K et al. (1996) The pathogenesis of gonococcal urethritis in men: confocal and immunoelectron microscopic analysis of urethral exudates from men infected with Neisseria gonorrhoeae. J Infect Dis 173:636-46
Porat, N; Apicella, M A; Blake, M S (1995) Neisseria gonorrhoeae utilizes and enhances the biosynthesis of the asialoglycoprotein receptor expressed on the surface of the hepatic HepG2 cell line. Infect Immun 63:1498-506
Porat, N; Apicella, M A; Blake, M S (1995) A lipooligosaccharide-binding site on HepG2 cells similar to the gonococcal opacity-associated surface protein Opa. Infect Immun 63:2164-72
Blake, M S; Blake, C M; Apicella, M A et al. (1995) Gonococcal opacity: lectin-like interactions between Opa proteins and lipooligosaccharide. Infect Immun 63:1434-9
Brooks, G F; Lammel, C J; Blake, M S et al. (1992) Antibodies against IgA1 protease are stimulated both by clinical disease and asymptomatic carriage of serogroup A Neisseria meningitidis. J Infect Dis 166:1316-21
Wetzler, L M; Blake, M S; Barry, K et al. (1992) Gonococcal porin vaccine evaluation: comparison of Por proteosomes, liposomes, and blebs isolated from rmp deletion mutants. J Infect Dis 166:551-5
Koomey, M; Bergstrom, S; Blake, M et al. (1991) Pilin expression and processing in pilus mutants of Neisseria gonorrhoeae: critical role of Gly-1 in assembly. Mol Microbiol 5:279-87
Butler, C A; Gotschlich, E C (1991) High-frequency mobilization of broad-host-range plasmids into Neisseria gonorrhoeae requires methylation in the donor. J Bacteriol 173:5793-9
Blake, M S; Eastby, C (1991) Studies on the gonococcal IgA1 protease II. Improved methods of enzyme purification and production of monoclonal antibodies to the enzyme. J Immunol Methods 144:215-21
Blake, M S; MacDonald, C M; Klugman, K P (1989) Colony morphology of piliated Neisseria meningitidis. J Exp Med 170:1727-36

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