Abstract

The long range goal of these studies is to provide a detailed understanding of the mechanism by which macrophages process and present antigen to lymphocytes and how H-2I gene function is expressed at the macrophage level. The strategy will be to focus on protocols designed to define and isolate the antigen recognition phase of lymphocyte activation. Experiments will be designed to test the hypothesis that antigen processing represents the catabolism of antigen by macrophages via a phagosome-lysosome pathway. Monoclonal antibodies and antigen specific T cell lines will be used as probes for macrophage associated antigen, and a novel method for determining which components of an infectious agent (Listeria monocytogenes) are most important for inducing protective immunity will be investigated. This will involve the identification of antigenic determinants selectively expressed on the macrophage plasma membrane following antigen processing.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020215-04
Application #
3129732
Study Section
Experimental Immunology Study Section (EI)
Project Start
1983-08-01
Project End
1987-09-29
Budget Start
1986-08-01
Budget End
1987-09-29
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Emory University
Department
Type
Schools of Medicine
DUNS #
042250712
City
Atlanta
State
GA
Country
United States
Zip Code
30322

  Publications

Miller, Mark A; Skeen, Marianne J; Lavine, Christy L et al. (2003) IL-12-assisted immunization generates CD4+ T cell-mediated immunity to Listeria monocytogenes. Cell Immunol 222:1-14
Hiltbold, E M; Ziegler, H K (1996) Interferon-gamma and interleukin-10 have cross-regulatory roles in modulating the class I and class II MHC-mediated presentation of epitopes of Listeria monocytogenes by infected macrophages. J Interferon Cytokine Res 16:547-54
Skeen, M J; Miller, M A; Shinnick, T M et al. (1996) Regulation of murine macrophage IL-12 production. Activation of macrophages in vivo, restimulation in vitro, and modulation by other cytokines. J Immunol 156:1196-206
Hiltbold, E M; Safley, S A; Ziegler, H K (1996) The presentation of class I and class II epitopes of listeriolysin O is regulated by intracellular localization and by intercellular spread of Listeria monocytogenes. J Immunol 157:1163-75
Skeen, M J; Ziegler, H K (1995) Activation of gamma delta T cells for production of IFN-gamma is mediated by bacteria via macrophage-derived cytokines IL-1 and IL-12. J Immunol 154:5832-41
Safley, S A; Jensen, P E; Reay, P A et al. (1995) Mechanisms of T cell epitope immunodominance analyzed in murine listeriosis. J Immunol 155:4355-66
Verma, N K; Ziegler, H K; Stocker, B A et al. (1995) Induction of a cellular immune response to a defined T-cell epitope as an insert in the flagellin of a live vaccine strain of Salmonella. Vaccine 13:235-44
Verma, N K; Ziegler, H K; Wilson, M et al. (1995) Delivery of class I and class II MHC-restricted T-cell epitopes of listeriolysin of Listeria monocytogenes by attenuated Salmonella. Vaccine 13:142-50
Skeen, M J; Ziegler, H K (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection. J Exp Med 178:971-84
Skeen, M J; Ziegler, H K (1993) Intercellular interactions and cytokine responsiveness of peritoneal alpha/beta and gamma/delta T cells from Listeria-infected mice: synergistic effects of interleukin 1 and 7 on gamma/delta T cells. J Exp Med 178:985-96

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