Abstract

The purpose of this proposal is to identify and characterize bacterial surface groups that react in a selective way with the Fc-regions of a variety of different immunoglobulin classes and subclasses from various mammals.
The aim i s to obtain molecules with reactivities differing from those of staphylococcal protein A which can then be used alone of in combination with protein A for a wide variety of selective immunoassays and in the purification of specific immunoglobulin classes and subclasses. The role of bacterial Fc-reactive proteins in the disease course and post-infection sequelae of certain infections will be explored as suitable reagents become available. In particular we propose to study the potential role of bacterial Fc-reactive proteins in the pathogenesis of post-streptococcal glomerulonephritis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020445-03
Application #
3130129
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1983-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Florida
Department
Type
Schools of Medicine
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

Podbielski, A; Weber-Heynemann, J; Cleary, P P (1994) Immunoglobulin-binding FcrA and Enn proteins and M proteins of group A streptococci evolved independently from a common ancestral protein. Med Microbiol Immunol 183:33-42
Raeder, R; Boyle, M D (1993) Association of type II immunoglobulin G-binding protein expression and survival of group A streptococci in human blood. Infect Immun 61:3696-702
Raeder, R; Otten, R A; Chamberlin, L et al. (1992) Functional and serological analysis of type II immunoglobulin G-binding proteins expressed by pathogenic group A streptococci. J Clin Microbiol 30:3074-81
Otten, R A; Raeder, R; Heath, D G et al. (1992) Identification of two type IIa IgG-binding proteins expressed by a single group A streptococcus. J Immunol 148:3174-82
Otten, R A; Boyle, M D (1991) The mitogenic activity of type III bacterial Ig binding proteins (protein G) for human peripheral blood lymphocytes is not related to their ability to react with human serum albumin or IgG. J Immunol 146:2588-95
Raeder, R; Faulmann, E L; Boyle, M D (1991) Evidence for functional heterogeneity in IgG Fc-binding proteins associated with group A streptococci. J Immunol 146:1247-53
Coleman, S E; Brady, L J; Boyle, M D (1990) Colloidal gold immunolabeling of immunoglobulin-binding sites and beta antigen in group B streptococci. Infect Immun 58:332-40
Faulmann, E L; Otten, R A; Barrett, D J et al. (1989) Immunological applications of type III Fc binding proteins. Comparison of different sources of protein G. J Immunol Methods 123:269-81
Brady, L J; Boyle, M D (1989) Identification of non-immunoglobulin A-Fc-binding forms and low-molecular-weight secreted forms of the group B streptococcal beta antigen. Infect Immun 57:1573-81
Yarnall, M; Boyle, M D (1986) Isolation and partial characterization of a type II Fc receptor from a group A streptococcus. Mol Cell Biochem 70:57-66

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