This is a proposal to study the immunobiology of the major histocompatibility complex, particularly that of I-region gene products. Peptide antigens will be synthesized and the specificity of the T cell immune response to these will be determined. The effects of varying the immunogen and the MHC-type of the antigen-presenting cell will be determined. These studies will be useful in defining whether the MHC-gene products act as part of the antigen receptor complex; if so, determining their specificity and the relationship of that specificity to immune response (Ir) gene effects. In order to accomplish these studies, T cell hybridomas will be constructed and the specificity of their antigen dependent IL-2 production assayed. The fusion of two such hybridomas to look for complementation of MHC-and antigen-receptors, when their putative antigen/Ia interactions are fixed, will be attempted.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020661-03
Application #
3130455
Study Section
Immunobiology Study Section (IMB)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Fox Chase Cancer Center
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19111