The purpose of this investigation is to gain understanding of the interrelationships between various elements of the immune response to respiratory viruses which lead to protective or to harmful effects. The study will develop immunizing strategies to Sendai virus and respiratory syncytical virus which will evoke a response to only one or two antigens of these viruses. This will be accomplished by isolating and purifying the surface glycoproteins of the viruses and reconstituting them separately or in combination into lipid vesicles. The strategies will evoke the restricted antibody responses either in the serum or in the secretions or both as well as evoking systemic and local cell-mediated immunity. This will be done by immunizing the animals either parenterally or through the mucosa, each route with and without adjuvants. Animals immunized with these strategies will have different profiles of response to the viruses. Such animals with different responses will be challenged by the respiratory route with live virus and their response to the challenge observed. The degree of protection or harm will be ascertained by quantifying the amount of virus shedding in the challenged animals, determining the type of pathology and observing the morbidity and mortality among the animals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI020925-03
Application #
3130745
Study Section
Virology Study Section (VR)
Project Start
1985-09-30
Project End
1988-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
State University of New York at Buffalo
Department
Type
School of Medicine & Dentistry
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260