Abstract

The immediate aims of this project are to identify those bacterial factors which are both necessary and sufficient for E. coli to colonize the mouse urinary tract in an ascending model of pyelonephritis. The investigators experiments to date indicate that P and type 1 fimbriae and the polysaccharide antigens O and K are necessary for E. coli to successfully colonize the mouse urinary tract. They now propose to determine which of these factors are sufficient for pathogenesis by transferring genes or gene clusters for these different traits singly and in combination into an E. coli K12 strain. The ability of these recombinants to resist serum killing and to colonize the urinary tract of mice will be assessed. The investigators also propose to further evaluate the biological characteristics of three different types of P-fimbriae by cloning the operons for each type of fimbria into a fecally-derived strain of E. coli that is otherwise nonpathogenic in the mouse model.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI021009-09
Application #
3130906
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Project Start
1984-07-01
Project End
1996-07-31
Budget Start
1993-08-01
Budget End
1994-07-31
Support Year
9
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Klann, A G; Hull, R A; Palzkill, T et al. (1994) Alanine-scanning mutagenesis reveals residues involved in binding of pap-3-encoded pili. J Bacteriol 176:2312-7
Hull, R A; Nowicki, B; Kaul, A et al. (1994) Effect of pap copy number and receptor specificity on virulence of fimbriated Escherichia coli in a murine urinary tract colonization model. Microb Pathog 17:79-86
Batchelor, R A; Alifano, P; Biffali, E et al. (1992) Nucleotide sequences of the genes regulating O-polysaccharide antigen chain length (rol) from Escherichia coli and Salmonella typhimurium: protein homology and functional complementation. J Bacteriol 174:5228-36
Klann, A G; Hull, R A; Hull, S I (1992) Sequences of the genes encoding the minor tip components of Pap-3 pili of Escherichia coli. Gene 119:95-100
Ding, M J; Svanborg, C; Haraguchi, G E et al. (1991) Molecular cloning and expression of the 01 rfb region from a pyelonephritic Escherichia coli 01:H1:K7. Microb Pathog 11:379-85
Batchelor, R A; Haraguchi, G E; Hull, R A et al. (1991) Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli. J Bacteriol 173:5699-704
Haraguchi, G E; Zahringer, U; Jann, B et al. (1991) Genetic characterization of the O4 polysaccharide gene cluster from Escherichia coli. Microb Pathog 10:351-61
Nowicki, B; Runyan, R S; Smith, N et al. (1990) Kinetics of colonization of a porous vitreous carbon percutaneous implant. Biomaterials 11:389-92
Karr, J F; Nowicki, B J; Truong, L D et al. (1990) pap-2-encoded fimbriae adhere to the P blood group-related glycosphingolipid stage-specific embryonic antigen 4 in the human kidney. Infect Immun 58:4055-62
Nowicki, B; Labigne, A; Moseley, S et al. (1990) The Dr hemagglutinin, afimbrial adhesins AFA-I and AFA-III, and F1845 fimbriae of uropathogenic and diarrhea-associated Escherichia coli belong to a family of hemagglutinins with Dr receptor recognition. Infect Immun 58:279-81

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