The purpose of this application is to study the activation of resting, antigen-specific virgin and memory B cells by two types of helper T cells (Th1 and Th2). The first part of the proposal will investigate the events occurring during """"""""conjugation"""""""" of antigen-specific B cells (that have processed antigen) and The cells. These events will include the identification of gap junctions, and the transfer of mediators and/or lymphokines between the two cells. In addition, we will determine if only those antigen-specific B cells that conjugate to the T cells in an antigen-specific, MHC-restricted fashion differentiate into antibody-secreting cells. The second part of the application will address the question of which B cells are activated by each type of The cell (Th1 and Th2) and the role of T cell-derived lymphokines produced by these The cells during T/B interaction. Finally, we will test a model of T/B interaction leading to B cell activation by adding, sequentially, antigen, different types of antigen-specific The cells (Th1 or Th2) or purified lymphokines (IL-4, IL-2, gamma-IFN, etc.) to resting, antigen-specific B cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI021229-08
Application #
3131161
Study Section
Immunobiology Study Section (IMB)
Project Start
1984-07-01
Project End
1992-11-30
Budget Start
1991-12-01
Budget End
1992-11-30
Support Year
8
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Type
Schools of Medicine
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390
Lee, W T; Vitetta, E S (1992) Changes in expression of CD45R during the development of Th1 and Th2 cell lines. Eur J Immunol 22:1455-9
Yin, X M; Vitetta, E S (1992) The lineage relationship between virgin and memory B cells. Int Immunol 4:691-8
Lee, W T; Vitetta, E S (1992) Memory T cells are anergic to the superantigen staphylococcal enterotoxin B. J Exp Med 176:575-9
Berton, M T; Vitetta, E S (1992) IL-4-induced expression of germline gamma 1 transcripts in B cells following cognate interactions with T helper cells. Int Immunol 4:387-96
Yefenof, E; Ela, C; Kotler, M et al. (1992) Induction of IL-4 secretion by the radiation leukemia virus (RadLV): role in autocrine growth stimulation of RadLV infected pre-leukemic cells. Int J Cancer 50:481-5
Sanders, V M; Vitetta, E S (1991) B cell-associated LFA-1 and T cell-associated ICAM-1 transiently cluster in the area of contact between interacting cells. Cell Immunol 132:45-55
Fernandez-Botran, R (1991) Soluble cytokine receptors: their role in immunoregulation. FASEB J 5:2567-74
Yin, X M; Vitetta, E S (1991) IL-4 down-regulates the expression of J11d on murine B cells. Cell Immunol 137:461-73
Burger, C; Vitetta, E S (1991) The response of B cells in spleen, Peyer's patches, and lymph nodes to LPS and IL-4. Cell Immunol 138:35-43
Fernandez-Botran, R; Vitetta, E S (1991) Evidence that natural murine soluble interleukin 4 receptors may act as transport proteins. J Exp Med 174:673-81

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