Abstract

Nickel is a biologically active trace metal important to mammalian health, as are numerous obligately anaerobic bacteria which inhabit the microintestinal environment. The proposed study centers on further resolving i) the biochemical/biological nature of nickel in acetogenic bacteria, and ii) the autotrophic metabolism of this biological group. Specifically, we plan to continue our studies on the biochemistry and physiological function of the nickel enzyme, carbon monoxide dehydrogenase, and to characterize acetogenic hydrogenase. Further analyses on the regulation of these enzymes will yield insights into their physiological roles, and antibodies (rabbit) against these catalysts will be used as specific probes to aid in this elucidation. Additionally, purification of recently discovered metallo nickel proteins (currently of unknown function) will be undertaken, as will studies on nickel transport and the genetics of acetogenic bacteria. A recently developed minimally defined medium will facilitate this latter work. Since acetogens are obligate anaerobes and are sensitive to oxidation, anaerobic cultivation and purification procedures will be utilized throughout the investigation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI021852-04
Application #
3132269
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1984-12-01
Project End
1989-11-30
Budget Start
1987-12-01
Budget End
1988-11-30
Support Year
4
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Mississippi
Department
Type
Schools of Arts and Sciences
DUNS #
City
University
State
MS
Country
United States
Zip Code
38677

  Publications

Lux, M F; Drake, H L (1992) Re-examination of the metabolic potentials of the acetogens Clostridium aceticum and Clostridium formicoaceticum: chemolithoautotrophic and aromatic-dependent growth. FEMS Microbiol Lett 74:49-56
Daniel, S L; Keith, E S; Yang, H et al. (1991) Utilization of methoxylated aromatic compounds by the acetogen Clostridium thermoaceticum: expression and specificity of the co-dependent O-demethylating activity. Biochem Biophys Res Commun 180:416-22
Hsu, T; Daniel, S L; Lux, M F et al. (1990) Biotransformations of carboxylated aromatic compounds by the acetogen Clostridium thermoaceticum: generation of growth-supportive CO2 equivalents under CO2-limited conditions. J Bacteriol 172:212-7
Daniel, S L; Hsu, T; Dean, S I et al. (1990) Characterization of the H2- and CO-dependent chemolithotrophic potentials of the acetogens Clostridium thermoaceticum and Acetogenium kivui. J Bacteriol 172:4464-71
Yang, H C; Drake, H L (1990) Differential effects of sodium on hydrogen- and glucose-dependent growth of the acetogenic bacterium Acetogenium kivui. Appl Environ Microbiol 56:81-6
Lux, M F; Keith, E; Hsu, T D et al. (1990) Biotransformations of aromatic aldehydes by acetogenic bacteria. FEMS Microbiol Lett 55:73-7
Hsu, T D; Lux, M F; Drake, H L (1990) Expression of an aromatic-dependent decarboxylase which provides growth-essential CO2 equivalents for the acetogenic (Wood) pathway of Clostridium thermoaceticum. J Bacteriol 172:5901-7
Yang, H C; Daniel, S L; Hsu, T D et al. (1989) Nickel transport by the thermophilic acetogen Acetogenium kivui. Appl Environ Microbiol 55:1078-81
Lundie Jr, L L; Yang, H C; Heinonen, J K et al. (1988) Energy-dependent, high-affinity transport of nickel by the acetogen Clostridium thermoaceticum. J Bacteriol 170:5705-8
Bryson, M F; Drake, H L (1988) Energy-dependent transport of nickel by Clostridium pasteurianum. J Bacteriol 170:234-8

Showing the most recent 10 out of 13 publications