1. We produce transgenic mice which express human class 1 major histocompatibility complex (MHC) gene, HLA-A2 and HLA-B7, by egg microinjection techniques. 2. We study gene integration and gene expression in such transgenic mice by Southern and Northern blot hybridization, immunological assays and two-dimensional protein gel analysis. The inheritance of the human HLA gene is also studied. 3. We study the function of HLA antigens in the transgenic mice. We generate cytotoxic T cell responses using human influenza virus, H-Y antigen and dinitrophenyl haptens in transgenic mice and determine the pattern of restriction specificity. 4. We study the tolerance against the HLA antigens in transgenic mice in skin graft, mixed lymphocyte reaction (MLR) and cytotoxic T cell responses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI022075-02
Application #
3132752
Study Section
Immunobiology Study Section (IMB)
Project Start
1985-03-01
Project End
1987-02-28
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost
Name
City of Hope National Medical Center
Department
Type
DUNS #
City
Duarte
State
CA
Country
United States
Zip Code
91010