Abstract

This project will isolate and characterize T-cell products which may be involved in antigen recognition. T cell proteins which bind antigen will be isolated from specifically sensitized T cells by absorbtion to and elution from antigen immunoabsorbents. Isolated antigen-binding molecules will be characterized by polyacrylamide gel electorphoresis, isoelectric focussing and peptide mapping. The expression of these molecules by T cell subsets will be determined. Antisera to antigen-binding T cell proteins will be prepared to study the function of these molecules in antigen recognition.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI022869-02
Application #
3134483
Study Section
(SSS)
Project Start
1985-09-30
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Connecticut
Department
Type
School of Medicine & Dentistry
DUNS #
City
Farmington
State
CT
Country
United States
Zip Code
06030

  Publications

Cone, R E (1992) Soluble T lymphocyte antigen-specific molecules. Proc Soc Exp Biol Med 200:296-302
Urbanski, M; Cone, R E (1992) Appearance of T lymphocyte-derived proteins specific for the immunizing antigen in serum during a humoral immune response. J Immunol 148:2840-4
Cone, R E; Weischedel, A K; Urbanski, M et al. (1992) Specific antigen binding by proteins secreted by an antigen-specific T cell hybrid. Mol Immunol 29:689-96
Cone, R E; Clark, R B; Kristie, J et al. (1991) T cell non-MHC-restricted antigen-binding molecules secreted or associated with the cell membrane are antigenically distinct. Cell Immunol 137:529-38
DiBrino, M; Ravindran, B; Cone, R E (1991) T cell derived proteins from normal human sera and their relationship to T cell antigen binding molecules. Clin Immunol Immunopathol 59:271-87
Rellahan, B L; Cone, R E (1990) Ontogeny and expression of non-MHC-restricted T cell antigen-binding molecules by thymocytes and peripheral T cell subsets. Cell Immunol 130:176-85
Rellahan, B L; Cone, R E (1989) Expression of non-MHC-restricted T cell antigen-binding molecules by thymic lymphocytes. Cell Immunol 123:166-76
Hubbard, R A; Speidel, M T; Marchalonis, J J et al. (1989) A monoclonal antigen-binding T cell immunoprotein: antigenic relatedness to T cell receptor beta chain FR1 V and J peptide segments: physicochemical distinctiveness from classical immunoglobulins and T cell receptor heterodimers. Mol Immunol 26:447-56
Cone, R E; Zheng, H G; Chue, B et al. (1988) T cell-derived antigen binding molecules (TABM): molecular and functional properties. Int Rev Immunol 3:205-28
Cone, R E; Gerardi, D A; Davidoff, J et al. (1987) Quantitation of T cell antigen-binding molecules (TABM) in the sera of nonimmunized, immunized, and desensitized mice. J Immunol 138:234-9

Showing the most recent 10 out of 13 publications