Abstract

These studies are aimed at understanding the developmental pathways and functional heterogeneity of different mouse B lymphocyte subpopulations. Lymphocytes are being classified according to surface phenotype using monoclonal antibodies and fluorescence-activated cell sorter analysis, and according to function using antigen-dependent B cell activation in an in vitro serum-free defined culture system. In addition, cloned T cell lines are being used to activate B-cell immunoglobulin synthesis in order to determine the number of receptors on B cells for T lymphocyte helper factors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI022871-02
Application #
3134491
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1985-04-01
Project End
1988-03-31
Budget Start
1986-04-01
Budget End
1988-03-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Medical Biology Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Rochford, R; Mosier, D E (1995) Differential Epstein-Barr virus gene expression in B-cell subsets recovered from lymphomas in SCID mice after transplantation of human peripheral blood lymphocytes. J Virol 69:150-5
Van Kuyk, R; Mosier, D E (1995) Lack of pseudotype formation between human immunodeficiency virus type 1 and Epstein-Barr virus in productively coinfected B lymphoblastoid cell lines. Virology 209:643-8
Riggs, J E; Feeney, A J; Kirven, M et al. (1994) VH11 bias and normal V-D-J junctions in SCID B lymphocytes rescued by neonatal T cell transfer. Mol Immunol 31:783-91
Picchio, G R; Cohen, J I; Wyatt, E R et al. (1993) Enhanced tumorigenicity of an Epstein-Barr virus-transformed lymphoblastoid cell line is associated with a unique 1:18 chromosomal translocation and decreased expression of lymphocyte function associated antigen-1a (CD11A). Am J Pathol 143:342-9
Mosier, D E; Stell, K L; Gulizia, R J et al. (1993) Homozygous scid/scid;beige/beige mice have low levels of spontaneous or neonatal T cell-induced B cell generation. J Exp Med 177:191-4
Feeney, A J (1992) Comparison of junctional diversity in the neonatal and adult immunoglobulin repertoires. Int Rev Immunol 8:113-22
Cohen, J I; Picchio, G R; Mosier, D E (1992) Epstein-Barr virus nuclear protein 2 is a critical determinant for tumor growth in SCID mice and for transformation in vitro. J Virol 66:7555-9
Picchio, G R; Kobayashi, R; Kirven, M et al. (1992) Heterogeneity among Epstein-Barr virus-seropositive donors in the generation of immunoblastic B-cell lymphomas in SCID mice receiving human peripheral blood leukocyte grafts. Cancer Res 52:2468-77
Riggs, J E; Hobbs, M V; Mosier, D E (1992) CD4+CD8- thymocytes from neonatal mice induce IgM production in SCID mice. J Immunol 148:1389-95
Thangavelu, M; Snyder, L; Anastasi, J et al. (1992) Cytogenetic characterization of B-cell lymphomas from severe combined immunodeficiency disease mice given injections of lymphocytes from Epstein-Barr virus-positive donors. Cancer Res 52:4678-81

Showing the most recent 10 out of 17 publications