Abstract

Our main goals are to identify, characterize, and isolate Fasciola hepatica antigens which can be used initially for the immunodiagnosis of acute and chronic fascioliasis in experimentally infected mice and rabbits and apply this knowledge to infections in humans. These antigens will also be evaluated as immunodiagnostic reagents to predict success of chemotherapy in infected humans. The antigens will be isolated and applied to sensitive immunologic assays including ELISA, RIA, and EITB, as well as others which we have shown to be highly effective for the immunodiagnosis of fascioliasis using crude antigens, like gel diffusion and counterelectrophoresis (CEP). Monospecific polyclonal and monoclonal antibodies will be developed to these antigens and used in the development of in vitro antigen purification assays like the antigen capture ELISA and to detect circulating antigens. These antigen specific probes will also aid in the dissection and study of the expression juvenile, semimature and mature antigens on the surface of the fluke.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI022906-02
Application #
3134582
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1986-09-01
Project End
1988-01-15
Budget Start
1987-09-01
Budget End
1988-01-15
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Puerto Rico Mayaguez
Department
Type
Schools of Arts and Sciences
DUNS #
City
Mayaguez
State
PR
Country
United States
Zip Code
00681

  Publications

Fagbemi, B O; Hillyer, G V (1992) The purification and characterization of a cysteine protease of Fasciola gigantica adult worms. Vet Parasitol 43:223-32
Hillyer, G V; Soler de Galanes, M; Rodriguez-Perez, J et al. (1992) Use of the Falcon assay screening test--enzyme-linked immunosorbent assay (FAST-ELISA) and the enzyme-linked immunoelectrotransfer blot (EITB) to determine the prevalence of human fascioliasis in the Bolivian Altiplano. Am J Trop Med Hyg 46:603-9
Fagbemi, B O; Hillyer, G V (1991) Partial purification and characterisation of the proteolytic enzymes of Fasciola gigantica adult worms. Vet Parasitol 40:217-26
Hillyer, G V; Soler de Galanes, M (1991) Initial feasibility studies of the fast-ELISA for the immunodiagnosis of fascioliasis. J Parasitol 77:362-5
Hillyer, G V (1989) Fasciola hepatica:Sp2/0 (helminth:myeloma) hybridoma expressing parasite antigen. Am J Trop Med Hyg 41:674-9
Langley, R J; Hillyer, G V (1989) Detection of circulating parasite antigen in murine fascioliasis by two-site enzyme-linked immunosorbent assays. Am J Trop Med Hyg 41:472-8
Langley, R J; Hillyer, G V (1989) Detection of circulating immune complexes by the enzyme-linked immunosorbent assay in sera from cattle infected with Fasciola hepatica. J Parasitol 75:690-5
Rivera Marrero, C A; Santiago, N; Hillyer, G V (1988) Evaluation of immunodiagnostic antigens in the excretory-secretory products of Fasciola hepatica. J Parasitol 74:646-52
Santiago, N; Hillyer, G V (1988) Antibody profiles by EITB and ELISA of cattle and sheep infected with Fasciola hepatica. J Parasitol 74:810-8
Hillyer, G V; Taylor, D W (1988) Immunoprecipitation of Fasciola hepatica mRNA in vitro translation products using infection and hyperimmune sera. Am J Trop Med Hyg 38:547-52

Showing the most recent 10 out of 11 publications