The primary goal of these studies is to determine how the influenza virus proteins recognize each other during the assembly process that leads to the production of the infectious particles. This virus is found ubiquitously in nature, affecting man and animal and causing epidemics and pandemics of enormous proportions. The virus causes temporary debilitating illness with substantial economic losses to the business sector. Despite its importance in producing disease, its manner of replication and assembly are not entirely clear. This project deals with the major structural component of the virus, the membrane M1 protein with two main aims. The first deals with the phosphorylation of M1 and how the modification participates in or facilitates the distribution of M1 in subcellular compartments and at the site of assembly on plasma membranes.
The second aim deals with ascertaining the existence of certain subviral particles (M1-RNP) in the cell which may progress to the plasma membrane site where budding occurs. These studies will involve the use of chemical cross-linkers, isolation of components by density gradients and immunoprecipitation of viral proteins. Temperature sensitive mutants will be used and assayed at restrictive and permissive conditions. Vaccinia recombinants containing the gene of M1 or M1 and NP will be used to determine the effect of phosphorylation of M1 on assembly and association of M1-RNP as the viral proteins proceed to the site of assembly.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI023446-03
Application #
2062186
Study Section
Experimental Virology Study Section (EVR)
Project Start
1986-09-01
Project End
1991-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost
Name
New York College of Podiatric Medicine
Department
Other Basic Sciences
Type
Colleges of Podiatric Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10035
Morrison, D C; Lei, M G; Chen, T Y et al. (1992) Identification and characterization of mammalian cell membrane receptors for LPS-endotoxin. Adv Exp Med Biol 319:23-9
Perera, P Y; Chan, T Y; Morrison, D C et al. (1992) Detection and analysis of the 80-kd lipopolysaccharide receptor in macrophages derived from Lpsn and Lpsd mice. J Leukoc Biol 51:501-6