The long term objectives of the research are to improve understanding of the evolution and diversity of antibacterial resistance plasmids in globally dispersed populations of bacteria in order to improve understanding and control of the spread of antibacterial resistance genes.
The specific aims are to determine and computer file EcoRI fragment size values for resistance plasmids selected by resistance phenotype or randomly from an extensive series of isolate collections, to detect in this data further copies of previously recognized stable plasmids and further delineate their distributions, to detect and delineate distribution of additional stable plasmids and estimate their diversity, to develop methods for detecting and analyzing lesser relatedness between plasmids, to survey resistance plasmid prevalence, diversity and identity in fecal E. coli from untreated and unhospitalized humans in different parts of the world, to adapt and apply to data on fragment sizes of may isolates on one plasmid available statistical programs to reconstruct the plasmid's phylogenetic tree, and to detect and establish fine detail of regions involved in recombinational events that are significant for the spread of antibacterial resistance genes in globally dispersed populations of bacteria.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI023474-02
Application #
3135619
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1987-03-01
Project End
1992-02-28
Budget Start
1987-03-01
Budget End
1988-02-29
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02115
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Lee, K Y; Hopkins, J D; O'Brien, T F et al. (1991) Gly-238-Ser substitution changes the substrate specificity of the SHV class A beta-lactamases. Proteins 11:45-51
Hopkins, J D; Flores, A; del Pilar Pla, M et al. (1991) Nosocomial spread of an amikacin resistance gene on both a mobilized, nonconjugative plasmid and a conjugative plasmid. Antimicrob Agents Chemother 35:1605-11
Lester, S C; del Pilar Pla, M; Wang, F et al. (1990) The carriage of Escherichia coli resistant to antimicrobial agents by healthy children in Boston, in Caracas, Venezuela, and in Qin Pu, China. N Engl J Med 323:285-9
Lee, K Y; Hopkins, J D; Syvanen, M (1990) Direct involvement of IS26 in an antibiotic resistance operon. J Bacteriol 172:3229-36
Hopkins, J D; O'Brien, T F; Syvanen, M (1988) Functional and structural map of pLST1000: a multiresistance plasmid widely distributed in Enterobacteriaceae. Plasmid 20:163-6
Medeiros, A A; O'Brien, T F; Rosenberg, E Y et al. (1987) Loss of OmpC porin in a strain of Salmonella typhimurium causes increased resistance to cephalosporins during therapy. J Infect Dis 156:751-7